Facinelli B, Giovanetti E, Casolari C, Varaldo P E
Institute of Microbiology, University of Ancona Medical School, Italy.
J Clin Microbiol. 1994 Dec;32(12):2929-35. doi: 10.1128/jcm.32.12.2929-2935.1994.
On the basis of preliminary trials with 14 collection strains of Listeria, five lectins (Canavalia ensiformis, concanavalin A; Griffonia simplicifolia lectin I; Helix pomatia agglutinin; Ricinus communis agglutinin; and Triticum vulgaris wheat germ agglutinin) were selected to set up a microtiter agglutination assay. The lectin agglutination profiles of 174 clinical, food, and environmental strains of Listeria monocytogenes, Listeria innocua, and Listeria seeligeri were investigated. Data on the standard determination of the antigenic structure were available for clinical strains; nonclinical isolates were assigned to serogroup 1 or 4 with commercial antisera. The listeria-lectin interaction was related to serological type rather than species; in particular, the strains assigned to serogroup 1 or belonging to serovars 1/2a, 1/2b, 1/2c, 3a, 3b, and 7 were never agglutinated by G. simplicifolia lectin I. The five-lectin set proved to be capable of detecting differences between serologically identical isolates of L. monocytogenes. Of the 150 isolates of this species, 144 were distributed over 15 different lectin agglutination profiles and 6 autoagglutinated, the overall typeability being 96%. However, the profiles encountered among L. monocytogenes isolates were not randomly distributed. With strains assigned to serogroup 1 or belonging to serovars 1/2a, 1/2b, 1/2c, and 3b, the clinical isolates fell into only two of the eight patterns recorded overall; with strains of serogroup 4 and serovar 4b, food and environmental isolates were distributed over eight of the nine patterns found in total, while clinical isolates were distributed over five patterns. In a comparative study of 15 epidemiologically relevant isolates of L. monocytogenes from five distinct outbreaks, strains with identical phage types and/or DNA fingerprints displayed identical lectin profiles. The heterogeneity of agglutination profiles may form the basis of a new approach to L. monocytogenes typing.
基于对14株李斯特菌收集菌株的初步试验，选择了5种凝集素（刀豆球蛋白A、伴刀豆球蛋白A；简单型 Griffonia 凝集素I；苹果蜗牛凝集素；蓖麻凝集素；以及普通小麦麦胚凝集素）来建立微量滴定凝集试验。研究了174株临床、食品和环境来源的单核细胞增生李斯特菌、无害李斯特菌和斯氏李斯特菌的凝集素凝集谱。临床菌株有关于抗原结构标准测定的数据；非临床分离株用商业抗血清鉴定为血清群1或4。李斯特菌与凝集素的相互作用与血清型有关，而不是与菌种有关；特别是，鉴定为血清群1或属于血清型1/2a、1/2b、1/2c、3a、3b和7的菌株从未被简单型 Griffonia 凝集素I凝集。这五种凝集素组合被证明能够检测单核细胞增生李斯特菌血清学相同分离株之间的差异。在该菌种的150株分离株中，144株分布在15种不同的凝集素凝集谱中，6株自动凝集，总体分型能力为96%。然而，单核细胞增生李斯特菌分离株中遇到的谱型并非随机分布。对于鉴定为血清群1或属于血清型1/2a、1/2b、1/2c和3b的菌株，临床分离株仅落入总体记录的八种模式中的两种；对于血清群4和血清型4b的菌株，食品和环境分离株分布在总共发现的九种模式中的八种，而临床分离株分布在五种模式中。在对来自五次不同疫情的15株具有流行病学相关性的单核细胞增生李斯特菌分离株的比较研究中，具有相同噬菌体类型和/或DNA指纹的菌株显示出相同的凝集素谱型。凝集谱型的异质性可能构成单核细胞增生李斯特菌分型新方法的基础。
