Jarløv J O, Rosdahl V T, Yeo M, Marples R R
Staphylococcus Laboratory, Statens Seruminstitut, Copenhagen, Denmark.
J Med Microbiol. 1993 Oct;39(4):305-9. doi: 10.1099/00222615-39-4-305.
Methicillin-resistant Staphylococcus aureus (MRSA) isolates from a possible outbreak in Singapore (84) were examined together with all MRSA isolated in Denmark in 1986-1990 (58) and 14 distinct epidemic and 10 distinct single hospital strains from England and Wales. All 84 Singapore isolates were phage typed routinely and 52 isolates were further analysed together with the Danish isolates with an additional set of experimental phages and by lectin typing. The British strains, previously phage typed in the same way, were lectin typed. The following lectins were used: wheat-term agglutinin, soy-bean agglutinin, tomato lectin and Concanavalin-A. Routine phage typing of the Danish isolates showed that 41 isolates belonged to 19 different types; 17 isolates were non-typable (NT). Addition of experimental phage typing and lectin typing enhanced discrimination to 47 types. The 24 British strains could be divided into eight [corrected] "lectin types". Sixty-one of the isolates from Singapore were non-typable by phage typing; the remaining 23 strains belonged to five types. Further examination of 52 isolates with the experimental set of phages and by lectin typing gave 14 closely related types; 48% of these isolates belonged to only two types.
对从新加坡可能爆发疫情中分离出的耐甲氧西林金黄色葡萄球菌（MRSA）菌株（84株），以及1986年至1990年在丹麦分离出的所有MRSA菌株（58株），连同来自英格兰和威尔士的14株不同的流行菌株和10株不同的单医院菌株一起进行了检测。对所有84株新加坡分离株进行了常规噬菌体分型，52株分离株与丹麦分离株一起，用另一组实验噬菌体并通过凝集素分型进行了进一步分析。英国菌株此前已用相同方法进行了噬菌体分型，此次进行了凝集素分型。使用了以下凝集素：麦胚凝集素、大豆凝集素、番茄凝集素和刀豆球蛋白A。丹麦分离株的常规噬菌体分型显示，41株分离株属于19种不同类型；17株分离株无法分型（NT）。增加实验噬菌体分型和凝集素分型后，鉴别能力提高到47种类型。24株英国菌株可分为8种[已修正]“凝集素类型”。新加坡分离出的61株菌株通过噬菌体分型无法分型；其余23株菌株属于5种类型。用实验噬菌体组并通过凝集素分型对52株分离株进行的进一步检测得出14种密切相关的类型；这些分离株中有48%仅属于两种类型。
