人唾液过氧化物酶的色谱分离

Chromatographic separation of human salivary peroxidases.

作者信息

Mäkinen K K, Tenovuo J

出版信息

Acta Odontol Scand. 1976;34(3):141-50. doi: 10.3109/00016357609002561.

Abstract

A series of rapid and simple chromatographic purification procedures for peroxidase-like enzymes occurring in the human oral cavity is presented. Samples of whole saliva, parotid saliva, gingival exudate and various bacterial preparations contain peroxidases which were purified using molecular exclusion and ion exchange chromatography, and isoelectric focusing. Salivary lactoperoxidase can be easily separated from bacterial and leucocyte peroxidase activity by the methods presented.

摘要

本文介绍了一系列用于纯化人口腔中过氧化物酶样酶的快速简便的色谱纯化方法。全唾液、腮腺唾液、牙龈渗出液和各种细菌制剂样本中均含有过氧化物酶，这些过氧化物酶通过分子排阻色谱、离子交换色谱和等电聚焦进行纯化。通过本文介绍的方法，唾液中的乳过氧化物酶能够轻易地与细菌和白细胞过氧化物酶活性分离。