Berg T, Midtvedt T
Acta Pathol Microbiol Scand A. 1976 Sep;84(5):415-20. doi: 10.1111/j.1699-0463.1976.tb00135.x.
The activities of beta-glucuronidase, arylsulphatase A and acid DNAase were measured in homogenates from Kupffer cells and hepatocytes prepared from germ-free, monocontaminated and conventional rats. The cells were prepared from a liver cell suspension obtained by treating the perfused liver with collagenase. Kupffer cells from germ-free rats were found to have lower lysosomal enzyme activities than cells obtained from conventional rats. Monocontaminated animals (E. coli or Streptococcus pyogenes) showed intermediate activities. Our data indicate that the level of lysosomal enzymes in macrophages is a function of the endocytic activity of these cells.
在无菌、单菌污染和普通大鼠制备的库普弗细胞和肝细胞匀浆中，测定了β-葡萄糖醛酸酶、芳基硫酸酯酶A和酸性脱氧核糖核酸酶的活性。这些细胞是由用胶原酶处理灌注肝脏获得的肝细胞悬液制备的。发现无菌大鼠的库普弗细胞的溶酶体酶活性低于普通大鼠的细胞。单菌污染的动物（大肠杆菌或化脓性链球菌）表现出中等活性。我们的数据表明，巨噬细胞中溶酶体酶的水平是这些细胞内吞活性的一个函数。
