猴出血热病毒基因组3'末端的分子特征
Molecular characterization of the 3' terminus of the simian hemorrhagic fever virus genome.
作者信息
Godeny E K, Zeng L, Smith S L, Brinton M A
机构信息
Department of Veterinary Microbiology and Parasitology, Louisiana State University School of Veterinary Medicine, Baton Rouge 70803.
出版信息
J Virol. 1995 Apr;69(4):2679-83. doi: 10.1128/JVI.69.4.2679-2683.1995.
Abstract
The 3' end of the simian hemorrhagic fever virus (SHFV) single-stranded RNA genome was cloned and sequenced. Adjacent to the 3' poly(A) tract, we identified a 76-nucleotide noncoding region preceded by two overlapping reading frames (ORFs). The ultimate 3' ORF of the viral genome encodes the capsid protein, and the penultimate ORF encodes the smallest SHFV envelope protein. These two ORFs overlap each other by 26 nucleotides. Northern (RNA) blot hybridization analyses of cytoplasmic RNA extracts from SHFV-infected MA-104 cells with gene-specific probes revealed the presence of full-length genomic RNA as well as six subgenomic SHFV-specific mRNA species. The subgenomic mRNAs are 3' coterminal. In its virion morphology and size, genome structure and length, and replication strategy, SHFV is most similar to lactate dehydrogenase-elevating virus, equine arteritis virus, and porcine reproductive and respiratory syndrome virus.
摘要
克隆并测序了猴出血热病毒（SHFV）单链RNA基因组的3'末端。在3'聚腺苷酸序列附近，我们鉴定出一个76个核苷酸的非编码区，其前面有两个重叠的阅读框（ORF）。病毒基因组的最末端3' ORF编码衣壳蛋白，倒数第二个ORF编码最小的SHFV包膜蛋白。这两个ORF彼此重叠26个核苷酸。用基因特异性探针对SHFV感染的MA-104细胞的细胞质RNA提取物进行Northern（RNA）印迹杂交分析，结果显示存在全长基因组RNA以及六种亚基因组SHFV特异性mRNA。亚基因组mRNA在3'端共末端。在病毒体形态和大小、基因组结构和长度以及复制策略方面，SHFV与乳酸脱氢酶升高病毒、马动脉炎病毒和猪繁殖与呼吸综合征病毒最为相似。
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