Meulenberg J J, Hulst M M, de Meijer E J, Moonen P L, den Besten A, de Kluyver E P, Wensvoort G, Moormann R J
Central Veterinary Institute, Department of Virology, Lelystad, The Netherlands.
Arch Virol Suppl. 1994;9:441-8. doi: 10.1007/978-3-7091-9326-6_43.
Lelystad virus (LV) is an enveloped positive-stranded RNA virus, which causes abortions and respiratory disease in pigs. The complete nucleotide sequence of the genome of LV has been determined. This sequence is 15.1 kb in length and contains a poly(A) tail at the 3' end. Open reading frames that might encode the viral replicases (ORFs 1a and 1b), membrane-associated proteins (ORFs 2 to 6) and the nucleocapsid protein (ORF7) have been identified. Sequence comparisons have indicated that LV is distantly related to the coronaviruses and toroviruses and closely related to lactate dehydrogenase-elevating virus (LDV) and equine arteritis virus (EAV). A 3' nested set of six subgenomic RNAs is produced in LV-infected alveolar lung macrophages. These subgenomic RNAs contain a leader sequence, which is derived from the 5' end of the viral genome. Altogether, these data show that LV is closely related evolutionarily to LDV and EAV, both members of a recently proposed family of positive-stranded RNA viruses, the Arteriviridae.
莱利斯塔德病毒（LV）是一种有包膜的正链RNA病毒，可导致猪流产和呼吸道疾病。LV基因组的完整核苷酸序列已被确定。该序列长度为15.1 kb，3'端含有一个poly(A)尾。已鉴定出可能编码病毒复制酶的开放阅读框（ORF 1a和1b）、膜相关蛋白（ORF 2至6）以及核衣壳蛋白（ORF7）。序列比较表明，LV与冠状病毒和环曲病毒亲缘关系较远，与乳酸脱氢酶升高病毒（LDV）和马动脉炎病毒（EAV）亲缘关系密切。在感染LV的肺泡巨噬细胞中产生了一组由六个亚基因组RNA组成的3'嵌套序列。这些亚基因组RNA含有一个前导序列，该序列来源于病毒基因组的5'端。总之，这些数据表明LV在进化上与LDV和EAV密切相关，后两者都是最近提出的正链RNA病毒科——动脉炎病毒科的成员。
