Liu Z, Jacobson A M, Luthy R G
Department of Civil and Environmental Engineering, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213.
Appl Environ Microbiol. 1995 Jan;61(1):145-51. doi: 10.1128/aem.61.1.145-151.1995.
The principal objective of this study was to quantify the bioavailability of micelle-solubilized naphthalene to naphthalene-degrading microorganisms comprising a mixed population isolated from contaminated waste and soils. Two nonionic surfactants were used, an alkylethoxylate, Brij 30 (C12E4), and an alkylphenol ethoxylate, Triton X-100 (C8PE9.5). Batch experiments were used to evaluate the effects of aqueous, micellized nonionic surfactants on the microbial mineralization of naphthalene and salicylic acid, an intermediate compound formed in the pathway of microbial degradation of naphthalene. The extent of solubilization and biodegradation under aerobic conditions was monitored by radiotracer and spectrophotometric techniques. Experimental results showed that surfactant concentrations above the critical micelle concentration were not toxic to the naphthalene-degrading bacteria and that the presence of surfactant micelles did not inhibit mineralization of naphthalene. Naphthalene solubilized by micelles of Brij 30 or Triton X-100 in liquid media was bioavailable and degradable by the mixed culture of bacteria.
本研究的主要目的是量化胶束增溶萘对从受污染废物和土壤中分离出的混合菌群中萘降解微生物的生物利用度。使用了两种非离子表面活性剂，一种是烷基乙氧基化物Brij 30（C12E4），另一种是烷基酚乙氧基化物Triton X-100（C8PE9.5）。采用批次实验来评估水相、胶束化非离子表面活性剂对萘和水杨酸（萘微生物降解途径中形成的中间化合物）微生物矿化的影响。通过放射性示踪和分光光度技术监测有氧条件下的增溶和生物降解程度。实验结果表明，高于临界胶束浓度的表面活性剂浓度对萘降解细菌无毒，并且表面活性剂胶束的存在不会抑制萘的矿化。在液体培养基中由Brij 30或Triton X-100胶束增溶的萘对于细菌混合培养物是可生物利用且可降解的。
