School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, People's Republic of China.
Appl Microbiol Biotechnol. 2010 Sep;88(1):327-39. doi: 10.1007/s00253-010-2737-6. Epub 2010 Jul 6.
It is uncertain in some extent that organic compounds solubilized in micelles of a nonionic surfactant aqueous solution are bioavailable directly by the microbes in an extractive microbial transformation or biodegradation process. In this work, a dose-response method, where a bioequivalence concept is introduced to evaluate the synergic toxicity of the nonionic surfactants and the organic compounds, was applied to analyze the inhibition effect of organic compounds (naphthalene, phenyl ether, 2-phenylethanol, and 1-butanol) in nonionic surfactant Triton X-100 micelle aqueous solutions and Triton X-114 in aqueous solutions forming cloud point systems. Based on the result, a mole solubilization ratio of organic compounds in micelle was also determined, which consisted very well with those of classic semi-equilibrium dialysis experiments. The results exhibit that bioavailability of organic compounds solubilized in micelles to microbial cells is negligible, which provides a guideline for application of nonionic surfactant micelle aqueous solutions or cloud point systems as novel media for microbial transformations or biodegradations.
在一定程度上还不确定，溶解在非离子表面活性剂水溶液胶束中的有机化合物是否可以直接被萃取微生物转化或生物降解过程中的微生物生物利用。在这项工作中，应用了一种剂量反应方法，其中引入了生物等效性概念来评估非离子表面活性剂和有机化合物的协同毒性，以分析有机化合物（萘、苯醚、2-苯乙醇和 1-丁醇）在非离子表面活性剂 Triton X-100 胶束水溶液和形成浊点体系的 Triton X-114 水溶液中的抑制作用。基于该结果，还确定了有机化合物在胶束中的摩尔增溶比，其与经典半平衡透析实验的结果非常吻合。结果表明，溶解在胶束中的有机化合物对微生物细胞的生物利用度可以忽略不计，这为将非离子表面活性剂胶束水溶液或浊点体系作为微生物转化或生物降解的新型介质的应用提供了指导。
