用于通过聚合酶链反应（PCR）检测卵磷脂酶阳性芽孢杆菌属的特异性寡核苷酸引物。
Specific oligonucleotide primers for detection of lecithinase-positive Bacillus spp. by PCR.
作者信息
Schraft H, Griffiths M W
机构信息
Department of Food Science, University of Guelph, Ontario, Canada.
出版信息
Appl Environ Microbiol. 1995 Jan;61(1):98-102. doi: 10.1128/aem.61.1.98-102.1995.
Abstract
An assay based on the PCR has been developed to facilitate detection and identification of Bacillus cereus in foods. Three primers for the PCR have been designed within the sequence for cereolysin AB, a cytolytic determinant that encodes lecithin-hydrolyzing and hemolytic activities of B. cereus. With the PCR and hybridization, the specificity of the primers was tested with 39 isolates of the B. cereus group, with 17 other Bacillus spp., and with 21 non-Bacillus strains. Results demonstrate a high specificity of the three oligonucleotides for isolates of the B. cereus group. With a combined PCR-hybridization assay, the detection limit for B. cereus in artificially contaminated milk was 1 CFU/ml of milk.
摘要
已开发出一种基于聚合酶链反应（PCR）的检测方法，以促进食品中蜡样芽孢杆菌的检测和鉴定。针对cereolysin AB序列设计了三种用于PCR的引物，cereolysin AB是一种细胞溶解决定簇，编码蜡样芽孢杆菌的卵磷脂水解和溶血活性。通过PCR和杂交技术，用39株蜡样芽孢杆菌属菌株、17株其他芽孢杆菌属菌株和21株非芽孢杆菌菌株对引物的特异性进行了测试。结果表明这三种寡核苷酸对蜡样芽孢杆菌属菌株具有高度特异性。采用PCR-杂交联合检测方法，人工污染牛奶中蜡样芽孢杆菌的检测限为每毫升牛奶1个菌落形成单位（CFU/ml）。
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