Algammal Abdelazeem M, Alfifi Khyreyah J, Mabrok Mahmoud, Alatawy Marfat, Abdel-Moneam Dalia A, Alghamdi Saad, Azab Marwa M, Ibrahim Reham A, Hetta Helal F, El-Tarabili Reham M
Department of Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt.
Biology Department, Faculty of Science, Tabuk University, Tabuk, 71421, Saudi Arabia.
Infect Drug Resist. 2022 Apr 24;15:2167-2185. doi: 10.2147/IDR.S365254. eCollection 2022.
is a common food poisoning pathogen in humans. This study aimed to investigate the prevalence, molecular typing, antibiogram profile, pathogenicity, dissemination of virulence and antibiotic resistance genes associated with natural infection among .
Consequently, 120 (40 healthy and 80 diseased) were obtained from private fish farms in Port-said Governorate, Egypt. Afterward, samples were processed for clinical, post-mortem, and bacteriological examinations. The recovered isolates were tested for antimicrobial susceptibility, phenotypic assessment of virulence factors, pathogeneicity, and PCR-based detection of virulence and antibiotic resistance genes.
was isolated from 30 (25%) examined fish; the highest prevalence was noticed in the liver (50%). The phylogenetic and sequence analyses of the B gene revealed that the tested isolate displayed a high genetic similarity with other strains from different origins. All the recovered isolates (n =60, 100%) exhibited β-hemolytic and lecithinase activities, while 90% (54/60) of the tested isolates were biofilm producers. Using PCR, the tested isolates harbor K, , and virulence genes with prevalence rates of 91.6%, 86.6%, 83.4%, 50%, and 33.4%, respectively. Moreover, 40% (24/60) of the tested isolates were multidrug-resistant (MDR) to six antimicrobial classes and carried the 1, 2, A, and A genes. The experimentally infected fish with showed variable mortality in direct proportion to the inoculated doses.
As far as we know, this is the first report that emphasized the existence of MDR in that reflects a threat to the public health and the aquaculture sector. Newly emerging MDR in commonly carried virulence genes K, and , as well as resistance genes 1, 2, A, and A.
是人类常见的食物中毒病原体。本研究旨在调查在自然感染中的流行情况、分子分型、抗菌谱、致病性、毒力传播及抗生素抗性基因。
因此，从埃及塞得港省的私人养鱼场获取了120份样本（40份健康样本和80份患病样本）。随后，对样本进行临床、尸检和细菌学检查。对分离出的菌株进行抗菌药敏试验、毒力因子表型评估、致病性检测以及基于聚合酶链反应（PCR）的毒力和抗生素抗性基因检测。
从30份（25%）检测的鱼中分离出该菌；在肝脏中检出率最高（50%）。对B基因的系统发育和序列分析表明，检测的分离株与来自不同来源的其他菌株具有高度遗传相似性。所有分离出的菌株（n = 60，100%）均表现出β - 溶血和卵磷脂酶活性，而90%（54/60）的检测菌株是生物膜产生菌。通过PCR检测，检测的菌株携带K、、和毒力基因，流行率分别为91.6%、86.6%、83.4%、50%和33.4%。此外，40%（24/60）的检测菌株对六种抗菌药物类别具有多重耐药性（MDR），并携带1、2、A和A基因。用该菌进行实验感染的鱼显示出与接种剂量成正比的不同死亡率。
据我们所知，这是第一份强调在中存在多重耐药菌的报告，这对公众健康和水产养殖业构成威胁。新出现的多重耐药菌通常携带毒力基因K、和，以及抗性基因1、2、A和A。
