Ahmed S P, Nash R J, Bridges C G, Taylor D L, Kang M S, Porter E A, Tyms A S
MRC Collaborative Centre, Mill Hill, London, UK.
Biochem Biophys Res Commun. 1995 Mar 8;208(1):267-73. doi: 10.1006/bbrc.1995.1333.
The 6-O-butanoyl derivative of castanospermine (MDL 28,574: BUCAST), an inhibitor of glycoprotein processing, blocked the growth of herpes simplex virus type-2 with the effect markedly enhanced by exposure of cells to the compound pre- as well as post-infection. The effectiveness of the derivative corresponded to an increased uptake with greatest accumulation after virus infection. Gas chromatography/mass spectrometry identified the predominant component in MDL 28,574 treated cells as castanospermine, an inhibitor of alpha-glucosidase 1. The effects of this compound on the synthesis of viral glycoprotein, gB, was determined with the increased molecular weight of the mannose-rich precursor evidence for the modulation of glycoprotein processing.
栗精胺的6 - O - 丁酰衍生物（MDL 28,574：丁酰栗精胺）是一种糖蛋白加工抑制剂，可阻断单纯疱疹病毒2型的生长，在感染前和感染后将细胞暴露于该化合物可显著增强这种效果。该衍生物的有效性与摄取增加相对应，在病毒感染后积累最多。气相色谱/质谱分析确定MDL 28,574处理细胞中的主要成分是α - 葡萄糖苷酶1的抑制剂栗精胺。通过富含甘露糖前体分子量的增加确定了该化合物对病毒糖蛋白gB合成的影响，这是糖蛋白加工受到调节的证据。
