Oram J D, Downing R G, Roff M, Serwankambo N, Clegg J C, Featherstone A S, Booth J C
Division of Pathology, Centre for Applied Microbiology and Research, Salisbury, Wiltshire, England.
AIDS Res Hum Retroviruses. 1991 Jul;7(7):605-14. doi: 10.1089/aid.1991.7.605.
Ugandan strains of human immunodeficiency virus type 1 (HIV-1) were isolated by cocultivation of peripheral blood lymphocytes from infected individuals with cord blood lymphocytes. Sequences from the V3 region of the env gene were amplified by the polymerase chain reaction (PCR) from chromosomal DNA obtained from low passage virus cultures. The PCR products from 13 Ugandan isolates were cloned into a phagemid vector and sequenced. Many isolates contained divergent V3 loop sequences and adjacent regions: diversity was associated with codon deletions or duplications and with nucleotide substitutions, especially G----A transitions. Proviruses from some of the cultures showed extensive diversity within the V3 loop sequences but others were more homogeneous. The V3 loop apices were conserved in 6 of the Ugandan proviruses and these were very similar to the equivalent regions of several Zairean proviruses. The V3 loop apices of African isolates of HIV-1 are divergent from those of North American isolates. The possible biological consequences of this divergence are discussed.
通过将感染个体的外周血淋巴细胞与脐血淋巴细胞共培养，分离出乌干达1型人类免疫缺陷病毒（HIV-1）毒株。从低传代病毒培养物获得的染色体DNA中，通过聚合酶链反应（PCR）扩增env基因V3区域的序列。将来自13株乌干达分离株的PCR产物克隆到噬菌粒载体中并进行测序。许多分离株含有不同的V3环序列和相邻区域：多样性与密码子缺失或重复以及核苷酸替换有关，尤其是G----A转换。一些培养物中的前病毒在V3环序列内显示出广泛的多样性，但其他的则更为均一。在6株乌干达前病毒中，V3环顶端是保守的，并且与几种扎伊尔前病毒的相应区域非常相似。HIV-1非洲分离株的V3环顶端与北美分离株不同。讨论了这种差异可能的生物学后果。
