Liotta L A, Kleinerman J, Saidel G M
Cancer Res. 1976 Sep;36(9 pt.1):3255-9.
Bacillus Calmette-Guérin (BCG) reduces the rate of spontaneous pulmonary metastases from a poorly immunogenic fibrosarcoma. To be effective, BCG (1 X 10(6) organisms) must be given in admixture with (1 X 10(6)) tumor cells at the time of transplantation. Reduction of metastasis at this dosage of BCG occurs without a change in the size of the primarytumor or the extent of necrosis within it. Tumors transplanted in admixture with spleen cells from BCG-exposed donors reduced the number of metastases, while spleen cells from normal or tumor-bearing donors had no effect on metastases. Fewer total tumor cells and clumps are collected from the venous effluent of tumors transplanted with BCG than from control tumors. The BCG-treated tumors have more host macrophages intimately associated with the effluent tumor cells then did controls. These data indicate that BCG can inhibit the metastatic potential of a weakly immunogenic fibrosarcoma. The mechanism of this effect appears to be a depression of entry or tumor cells into the tumor vascular channels which may be related to the interaction of tumor cells with BCG-stimulated macrophages.
卡介苗（BCG）可降低免疫原性较弱的纤维肉瘤自发肺转移的发生率。为达到有效效果，必须在移植时将BCG（1×10⁶个菌体）与（1×10⁶个）肿瘤细胞混合使用。在此剂量的BCG作用下，转移减少，而原发肿瘤的大小及其内部坏死程度均无变化。与来自接触过BCG供体的脾细胞混合移植的肿瘤，转移数量减少，而来自正常或荷瘤供体的脾细胞对转移没有影响。与对照肿瘤相比，从与BCG混合移植的肿瘤的静脉流出物中收集到的肿瘤细胞总数和细胞团更少。经BCG处理的肿瘤与对照相比，有更多的宿主巨噬细胞与流出的肿瘤细胞密切相关。这些数据表明，BCG可抑制弱免疫原性纤维肉瘤的转移潜能。这种作用机制似乎是抑制肿瘤细胞进入肿瘤血管通道，这可能与肿瘤细胞与BCG刺激的巨噬细胞之间的相互作用有关。
