Sykulev Y, Brunmark A, Jackson M, Cohen R J, Peterson P A, Eisen H N
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139.
Immunity. 1994 Apr;1(1):15-22. doi: 10.1016/1074-7613(94)90005-1.
We show here that the net rate of accumulation of complexes formed by the antigen-specific receptor of T cells (TCR) of a T cell clone with its natural ligand, an octapeptide in association with Ld, a class I protein of the major histocompatibility complex (MHC), approaches the maximal value determined by the affinity of the TCR for this peptide-MHC ligand in 1-2 min, which is well within the lifetime of transient T cell-target cell conjugates. Consistent with this finding, we also found that the widely divergent affinity values (equilibrium constants) of this TCR for six related peptide-MHC complexes correlate well with the extent of specific lysis of target cells bearing various level of these complexes.
我们在此表明，T细胞克隆的T细胞抗原特异性受体（TCR）与其天然配体（一种与主要组织相容性复合体（MHC）的I类蛋白Ld结合的八肽）形成的复合物的净积累速率在1 - 2分钟内接近由TCR对该肽 - MHC配体的亲和力所确定的最大值，这完全在短暂的T细胞 - 靶细胞结合物的寿命范围内。与此发现一致，我们还发现该TCR对六种相关肽 - MHC复合物的广泛不同的亲和力值（平衡常数）与携带不同水平这些复合物的靶细胞的特异性裂解程度密切相关。
