左氧氟沙星抗菌药物敏感性试验的解释标准与质量控制

Interpretive criteria and quality control for antimicrobial susceptibility tests of levofloxacin.

作者信息

Barry A, Fuchs P, Tenover F, Allen S, Hardy D, Jorgensen J, McLaughlin J, Reller L

机构信息

Clinical Microbiology Institute, Tualatin, Oregon 97062.

出版信息

Eur J Clin Microbiol Infect Dis. 1994 Oct;13(10):822-6. doi: 10.1007/BF02111343.

DOI:10.1007/BF02111343

PMID:7889952

Abstract

To confirm preliminary interpretive breakpoints for prototype 5 micrograms levofloxacin disks, 490 strains were tested in vitro using commercially manufactured disks. For in vitro susceptibility testing, 5 micrograms levofloxacin disks can be used with interpretive criteria of < or = 12 mm for resistant (MIC > or = 8.0 micrograms/ml) and > or = 16 mm for susceptible (MIC < or = 2.0 micrograms/ml). Proposed quality control limits for tests of levofloxacin are as follows: Escherichia coli ATCC 25922, zones 29-37 mm or MIC 0.008-0.03 microgram/ml; Pseudomonas aeruginosa ATCC 27853, zones 19-26 mm or MIC 0.5-2.0 micrograms/ml; Staphylococcus aureus ATCC 25923, zones 24-31 mm; Staphylococcus aureus ATCC 29213, MIC 0.06-0.25 microgram/ml and Enterococcus faecalis ATCC 29212, MIC 0.25-2.0 micrograms/ml.

摘要

为确定5微克左氧氟沙星原型药敏纸片的初步解释性断点，使用市售药敏纸片对490株菌株进行了体外测试。对于体外药敏试验，5微克左氧氟沙星药敏纸片可与以下解释标准一起使用：耐药（MIC≥8.0微克/毫升）时抑菌圈直径≤12毫米，敏感（MIC≤2.0微克/毫升）时抑菌圈直径≥16毫米。左氧氟沙星检测的建议质量控制限度如下：大肠埃希菌ATCC 25922，抑菌圈直径29 - 37毫米或MIC 0.008 - 0.03微克/毫升；铜绿假单胞菌ATCC 27853，抑菌圈直径19 - 26毫米或MIC 0.5 - 2.0微克/毫升；金黄色葡萄球菌ATCC 25923，抑菌圈直径24 - 31毫米；金黄色葡萄球菌ATCC 29213，MIC 0.06 - 0.25微克/毫升；粪肠球菌ATCC 29212，MIC 0.25 - 2.0微克/毫升。