• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Convenient method for detecting 14CO2 in multiple samples: application to rapid screening for mutants.

作者信息

Tabor H, Tabor C W, Hafner E W

出版信息

J Bacteriol. 1976 Oct;128(1):485-6. doi: 10.1128/jb.128.1.485-486.1976.

DOI:10.1128/jb.128.1.485-486.1976
PMID:789350
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC232878/
Abstract

A procedure is presented for the rapid screening of bacterial colonies to detect mutants unable to produce 14CO2 from a labeled precursor. The method is especially useful for mass screening for mutants that cannot be easily detected by their phenotypic characteristics.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7819/232878/3197c68e93e8/jbacter00311-0496-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7819/232878/3197c68e93e8/jbacter00311-0496-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7819/232878/3197c68e93e8/jbacter00311-0496-a.jpg

相似文献

1
Convenient method for detecting 14CO2 in multiple samples: application to rapid screening for mutants.
J Bacteriol. 1976 Oct;128(1):485-6. doi: 10.1128/jb.128.1.485-486.1976.
2
Mass screening for mutants in the biosynthetic pathway for polyamines in Escherichia coli: a general method for mutants in enzymatic reactions producing CO2.大肠杆菌中多胺生物合成途径突变体的大规模筛选：一种用于产生二氧化碳的酶促反应中突变体的通用方法。
Methods Enzymol. 1983;94:83-91. doi: 10.1016/s0076-6879(83)94014-4.
3
Escherichia coli mutants completely deficient in adenosylmethionine decarboxylase and in spermidine biosynthesis.完全缺乏腺苷甲硫氨酸脱羧酶和亚精胺生物合成的大肠杆菌突变体。
J Biol Chem. 1978 May 25;253(10):3671-6.
4
Quantitation of S-adenosylmethionine decarboxylase protein.S-腺苷甲硫氨酸脱羧酶蛋白的定量分析。
Biochemistry. 1985 Jul 30;24(16):4417-23. doi: 10.1021/bi00337a024.
5
Effect of aldehydes on polyamine metabolism. I. Method used to determine CO2 produced "in vitro" in enzymatic reactions: its application in evaluation of S-adenosylmethionine decarboxylase (SAMD) activity.醛类对多胺代谢的影响。I. 用于测定酶促反应中“体外”产生的二氧化碳的方法：其在评估S-腺苷甲硫氨酸脱羧酶（SAMD）活性中的应用。
Boll Soc Ital Biol Sper. 1981 Jun 15;57(11):1195-201.
6
Deletion mutations in the speED operon: spermidine is not essential for the growth of Escherichia coli.精胺合成酶操纵子中的缺失突变：亚精胺对大肠杆菌生长并非必需。
Gene. 1993 Apr 15;126(1):115-7. doi: 10.1016/0378-1119(93)90598-w.
7
An easy method for screening and isolating rod mutants of Bacillus subtilis.一种筛选和分离枯草芽孢杆菌杆状突变体的简便方法。
Appl Microbiol Biotechnol. 2004 May;64(4):551-5. doi: 10.1007/s00253-003-1489-y. Epub 2003 Dec 11.
8
An assay for the detection of superoxide dismutase in individual Escherichia coli colonies.
Anal Biochem. 1988 Feb 1;168(2):455-61. doi: 10.1016/0003-2697(88)90343-0.
9
Polyamine biosynthesis in Escherichia coli: construction of polyamine-deficient mutants.大肠杆菌中的多胺生物合成：多胺缺陷型突变体的构建
Med Biol. 1981 Dec;59(5-6):389-93.
10
S-adenosylmethionine decarboxylase (Escherichia coli).S-腺苷甲硫氨酸脱羧酶（大肠杆菌）
Methods Enzymol. 1983;94:228-30. doi: 10.1016/s0076-6879(83)94039-9.

引用本文的文献

1
A genetically encoded tool for manipulation of NADP/NADPH in living cells.一种用于在活细胞中操纵NADP/NADPH的基因编码工具。
Nat Chem Biol. 2017 Oct;13(10):1088-1095. doi: 10.1038/nchembio.2454. Epub 2017 Aug 7.
2
Probing the functional diversity of global pristine soil communities with 3-chlorobenzoate reveals that communities of generalists dominate catabolic transformation.利用 3-氯苯甲酸探测全球原始土壤群落的功能多样性表明，通才型群落主导着代谢转化。
Appl Environ Microbiol. 2013 Nov;79(22):6932-40. doi: 10.1128/AEM.01905-13. Epub 2013 Aug 30.
3
Pristine soils mineralize 3-chlorobenzoate and 2,4-dichlorophenoxyacetate via different microbial populations.

本文引用的文献

1
Multiple pathways of putrescine biosynthesis in Escherichia coli.大肠杆菌中腐胺生物合成的多种途径。
J Biol Chem. 1966 Jul 10;241(13):3129-35.
2
Purification of adenosylmethionine decarboxylase from Escherichia coli W: evidence for covalently bound pyruvate.从大肠杆菌W中纯化腺苷甲硫氨酸脱羧酶：共价结合丙酮酸的证据。
J Biol Chem. 1970 Apr 25;245(8):2132-9.
3
Urea production and putrescine biosynthesis by Escherichia coli.大肠杆菌的尿素生成与腐胺生物合成
原始土壤通过不同的微生物种群将3-氯苯甲酸酯和2,4-二氯苯氧基乙酸酯矿化。
Appl Environ Microbiol. 1996 Apr;62(4):1159-66. doi: 10.1128/aem.62.4.1159-1166.1996.
4
The ornithine decarboxylase gene odc is required for alcaligin siderophore biosynthesis in Bordetella spp.: putrescine is a precursor of alcaligin.鸟氨酸脱羧酶基因odc是博德特氏菌属中嗜铁素生物合成所必需的：腐胺是嗜铁素的前体。
J Bacteriol. 1996 Jan;178(1):54-60. doi: 10.1128/jb.178.1.54-60.1996.
5
S-adenosyl-L-methionine decarboxylase of Acanthamoeba castellanii (Neff): purification and properties.卡氏棘阿米巴（内夫株）的S-腺苷-L-甲硫氨酸脱羧酶：纯化及性质
Biochem J. 1993 Oct 1;295 ( Pt 1)(Pt 1):203-9. doi: 10.1042/bj2950203.
6
Genetics of the synthesis of serine from glycine and the utilization of glycine as sole nitrogen source by Saccharomyces cerevisiae.酿酒酵母从甘氨酸合成丝氨酸以及将甘氨酸用作唯一氮源的遗传学研究。
Genetics. 1995 Aug;140(4):1213-22. doi: 10.1093/genetics/140.4.1213.
7
Construction of an Escherichia coli strain unable to synthesize putrescine, spermidine, or cadaverine: characterization of two genes controlling lysine decarboxylase.构建无法合成腐胺、亚精胺或尸胺的大肠杆菌菌株：对两个控制赖氨酸脱羧酶的基因的表征
J Bacteriol. 1980 Dec;144(3):952-6. doi: 10.1128/jb.144.3.952-956.1980.
8
Linkage map of Escherichia coli K-12, edition 6.大肠杆菌K-12连锁图谱，第6版。
Microbiol Rev. 1980 Mar;44(1):1-56. doi: 10.1128/mr.44.1.1-56.1980.
9
Regulatory mutations affecting ornithine decarboxylase activity in Saccharomyces cerevisiae.影响酿酒酵母中鸟氨酸脱羧酶活性的调控突变
J Bacteriol. 1980 Jun;142(3):791-9. doi: 10.1128/jb.142.3.791-799.1980.
10
Effect of glucose on the amount of bacteria mineralizing 2,4-dichlorophenoxyacetic acid in soil.葡萄糖对土壤中矿化2,4-二氯苯氧乙酸的细菌数量的影响。
Folia Microbiol (Praha). 1983;28(1):54-6. doi: 10.1007/BF02877386.
J Bacteriol. 1967 Nov;94(5):1516-9. doi: 10.1128/jb.94.5.1516-1519.1967.
4
Mass screening for mutants with altered DNases by microassay techniques.通过微量测定技术对具有改变的脱氧核糖核酸酶的突变体进行大规模筛选。
Methods Enzymol. 1974;29:180-93. doi: 10.1016/0076-6879(74)29020-7.
5
Isolation, characterization, and mapping of Escherichia coli mutants blocked in the synthesis of ornithine decarboxylase.鸟氨酸脱羧酶合成受阻的大肠杆菌突变体的分离、特性鉴定及定位
J Bacteriol. 1975 Nov;124(2):791-9. doi: 10.1128/jb.124.2.791-799.1975.