Beacham I R, Taylor N S, Youell M
J Bacteriol. 1976 Nov;128(2):522-7. doi: 10.1128/jb.128.2.522-527.1976.
De novo synthesis of two periplasmic enzymes in Escherichia coli, alkaline phosphatase and acid hexose phosphatase, have been studied in the presence and absence of new phospholipid synthesis. Alkaline phosphatase synthesis was initiated by a temperature shift in a strain carrying a phoA amber mutation and a temperature-sensitive suppressor mutation; acid hexose phosphatase was studied after relief of catabolite repression. Glycerol auxotrophs (gpsA) were used to control phospholipid synthesis. Synthesis of both enzymes proceeded at a normal rate for 0.5 to 1.0 generation of growth, although it was then curtailed. It is concluded that secretion of these enzymes is not obligatorily coupled to new net phospholipid synthesis.
在有无新磷脂合成的情况下，对大肠杆菌中两种周质酶——碱性磷酸酶和酸性己糖磷酸酶的从头合成进行了研究。碱性磷酸酶的合成是通过携带phoA琥珀突变和温度敏感抑制突变的菌株中的温度转换启动的；在分解代谢物阻遏解除后研究了酸性己糖磷酸酶。甘油营养缺陷型（gpsA）用于控制磷脂合成。两种酶的合成在0.5至1.0代的生长时间内以正常速率进行，尽管随后受到了抑制。得出的结论是，这些酶的分泌并非必然与新的净磷脂合成相关联。
