Wainwright M, Beacham I R
Mol Gen Genet. 1977 Jul 7;154(1):67-73. doi: 10.1007/BF00265578.
Previous studies by others have indicated that the synthesis of secreted enzymes is unusually sensitive to many translation inhibitors and resistant, for about 30 min, to rifampicin. We have studied the sensitivity of secreted (periplasmic) phosphatases to such inhibitors. Alkaline phosphatase synthesis is more sensitive than total protein synthesis to tetracyclin and spectinomycin, but not to sparsomycin, streptomycin, chloramphenicol, kasugamycin, blasticidin S or thiostrepton; it is slightly more resistant than total protein synthesis to the latter two antibiotics. Acid hexose-phosphatase was also preferentially sensitive to tetracyclin and spectinomycin and also to kasugamycin. beta-galactosidase was also included in the study, as an intracellular enzyme, and was found to be preferentially inhibited ("repressed"), sometimes transiently, by all eight translation inhibitors. This effect did not seem to be mediated through cyclic AMP or guanosine tetraphosphate; the "repression" was still evident in mutants with altered rho factor indicating that it may also not be related to artificial polarity. Synthesis of both periplasmic phosphatases was immediately inhibited by rifampicin. These results differ from those found in previous studies with other organisms and suggest a reappraisal of the usual interpretation of these phenomena.
其他人之前的研究表明，分泌型酶的合成对许多翻译抑制剂异常敏感，并且在约30分钟内对利福平具有抗性。我们研究了分泌型（周质）磷酸酶对这类抑制剂的敏感性。碱性磷酸酶的合成比总蛋白合成对四环素和壮观霉素更敏感，但对稀疏霉素、链霉素、氯霉素、春日霉素、杀稻瘟菌素S或硫链丝菌素不敏感；它对后两种抗生素的抗性略高于总蛋白合成。酸性己糖磷酸酶也优先对四环素、壮观霉素以及春日霉素敏感。β-半乳糖苷酶作为一种胞内酶也被纳入研究，发现它会被所有八种翻译抑制剂优先抑制（“阻遏”），有时是短暂抑制。这种效应似乎不是通过环磷酸腺苷或四磷酸鸟苷介导的；在rho因子改变的突变体中“阻遏”仍然明显，这表明它可能也与人为极性无关。两种周质磷酸酶的合成均会被利福平立即抑制。这些结果与之前对其他生物体的研究结果不同，表明需要重新评估对这些现象的通常解释。
