Fujita Y, Uraga Y, Ichisima E
Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, Sendai, Japan.
Biochim Biophys Acta. 1995 Mar 14;1261(1):151-4. doi: 10.1016/0167-4781(95)00011-5.
The coding region of the protyrosinase gene, melO, from Aspergillus oryzae occupies 1671 base pairs of the genomic DNA and is separated into two exons by one intron. The full-length cDNA of the melO gene was cloned. Analysis of the 1617 base pairs nucleotide sequence revealed a single open reading frame coding 539 amino acid residues. The cDNA has been expressed in yeast cells. The predicted protein product derived from the melO gene is identified by Western blotting and activity determination. The predicted amino acid sequence of the gene product was compared with that of Neurospora crassa tyrosinase. A coupled pair of three histidine residues in the tyrosinase was assumed to correspond to Cu(II) ligands in the homologous tyrosinases from Streptomyces glaucescens and Homo sapiens.
来自米曲霉的原酪氨酸酶基因melO的编码区占据基因组DNA的1671个碱基对，并被一个内含子分隔成两个外显子。克隆了melO基因的全长cDNA。对1617个碱基对的核苷酸序列分析揭示了一个编码539个氨基酸残基的单一开放阅读框。该cDNA已在酵母细胞中表达。通过蛋白质免疫印迹法和活性测定鉴定了源自melO基因的预测蛋白质产物。将该基因产物的预测氨基酸序列与粗糙脉孢菌酪氨酸酶的氨基酸序列进行了比较。酪氨酸酶中一对相连的三个组氨酸残基被认为与来自浅绿链霉菌和智人的同源酪氨酸酶中的Cu(II)配体相对应。
