甜菜红素合成对转基因胡萝卜光合作用和酪氨酸代谢的影响。

Effect of betanin synthesis on photosynthesis and tyrosine metabolism in transgenic carrot.

机构信息

State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.

Shanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute, Shanghai Academy of Agricultural Science, Shanghai, 201106, China.

出版信息

BMC Plant Biol. 2023 Aug 24;23(1):402. doi: 10.1186/s12870-023-04383-9.

DOI:10.1186/s12870-023-04383-9

PMID:37620775

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10464428/

Abstract

BACKGROUND

Betalain is a natural pigment with important nutritional value and broad application prospects. Previously, we produced betanin biosynthesis transgenic carrots via expressing optimized genes CYP76AD1S, cDOPA5GTS and DODA1S. Betanin can accumulate throughout the whole transgenic carrots. But the effects of betanin accumulation on the metabolism of transgenic plants and whether it produces unexpected effects are still unclear.

RESULTS

The accumulation of betanin in leaves can significantly improve its antioxidant capacity and induce a decrease of chlorophyll content. Transcriptome and metabolomics analysis showed that 14.0% of genes and 33.1% of metabolites were significantly different, and metabolic pathways related to photosynthesis and tyrosine metabolism were markedly altered. Combined analysis showed that phenylpropane biosynthesis pathway significantly enriched the differentially expressed genes and significantly altered metabolites.

CONCLUSIONS

Results showed that the metabolic status was significantly altered between transgenic and non-transgenic carrots, especially the photosynthesis and tyrosine metabolism. The extra consumption of tyrosine and accumulation of betanin might be the leading causes.

摘要

背景

甜菜红素是一种具有重要营养价值和广泛应用前景的天然色素。此前，我们通过表达优化后的 CYP76AD1S、cDOPA5GTS 和 DODA1S 基因，生产出了甜菜红素生物合成的转基因胡萝卜。甜菜红素可以在整个转基因胡萝卜中积累。但是，甜菜红素的积累对转基因植物的代谢的影响，以及是否会产生意想不到的影响，目前尚不清楚。