Kristjánsson S, Shimizu T, Strannegård I L, Wennergren G
Department of Paediatrics, University of Gothenburg, Ostra Hospital, Sweden.
Pediatr Allergy Immunol. 1994 Nov;5(4):223-9. doi: 10.1111/j.1399-3038.1994.tb00244.x.
Serum levels of eosinophil cationic protein (ECP), myeloperoxidase (MPO), tryptase, total IgE and differential blood cell counts were studied in atopic children with: 1) moderate to severe asthma using inhaled steroids and symptom-free for the last 3 weeks (n = 13), 2) mild asthma with sporadic symptoms, using only inhaled beta 2-agonists < 3 times/week (n = 15), 3) acute asthmatic attacks admitted to hospital (n = 12), 4) mild to moderate atopic dermatitis (n = 14). Fifteen children without any history of atopy served as controls. ECP, MPO, tryptase and IgE were measured in serum by radioimmunoassays (RIA). The symptom-free children with inhaled steroids had similar median ECP and MPO values as the controls, 8.0 and 360 micrograms/l, vs. 9.0 and 310 micrograms/l, while both ECP and MPO were significantly (p < 0.001) increased in the symptom-free children without anti-inflammatory treatment, 32 and 887 micrograms/l and in those with acute asthma, 28 and 860 micrograms/l. The children with atopic dermatitis had increased ECP but normal MPO levels, 16.0 and 455 micrograms/l. Tryptase in serum was not measurable in any patient. All groups except the control group had significantly elevated total IgE levels. The results indicate that in atopic children serum ECP is a good marker of ongoing asthma or atopic dermatitis. The normal levels of ECP and MPO in the children with asthma using inhaled steroids seem to reflect successful anti-inflammatory treatment. The increased levels of ECP and MPO in the children with mild asthma and no anti-inflammatory treatment may indirectly reflect airway inflammation.
对患有以下疾病的特应性儿童的血清嗜酸性粒细胞阳离子蛋白（ECP）、髓过氧化物酶（MPO）、类胰蛋白酶、总IgE水平及血细胞分类计数进行了研究：1）使用吸入性类固醇且在过去3周无症状的中度至重度哮喘患儿（n = 13）；2）仅偶尔出现症状的轻度哮喘患儿，每周使用吸入性β2受体激动剂少于3次（n = 15）；3）因急性哮喘发作入院的患儿（n = 12）；4）轻度至中度特应性皮炎患儿（n = 14）。15名无任何特应性病史的儿童作为对照。采用放射免疫分析法（RIA）测定血清中的ECP、MPO、类胰蛋白酶和IgE。使用吸入性类固醇且无症状的儿童的ECP和MPO中位数与对照组相似，分别为8.0和360微克/升，而对照组为9.0和310微克/升；未接受抗炎治疗的无症状儿童以及急性哮喘患儿的ECP和MPO均显著升高（p < 0.001），分别为32和887微克/升以及28和860微克/升。特应性皮炎患儿的ECP升高但MPO水平正常，分别为16.0和455微克/升。所有患者血清中的类胰蛋白酶均无法检测到。除对照组外，所有组的总IgE水平均显著升高。结果表明，在特应性儿童中，血清ECP是当前哮喘或特应性皮炎的良好标志物。使用吸入性类固醇的哮喘患儿ECP和MPO水平正常似乎反映了抗炎治疗的成功。未接受抗炎治疗的轻度哮喘患儿ECP和MPO水平升高可能间接反映气道炎症。
