Kempe M, Mosbach K
Pure and Applied Biochemistry, University of Lund, Sweden.
J Chromatogr A. 1995 Feb 3;691(1-2):317-23. doi: 10.1016/0021-9673(94)00820-y.
Stationary phases, to be used in high-performance liquid chromatography, were tailor-made for the separation of amino acids, peptides and proteins. The stationary phases were prepared by molecular imprinting, applying two different approaches. Low-molecular-mass compounds were imprinted in bulk polymers by copolymerization of functional monomers and cross-linkers in the presence of the compound of interest, the print molecule. These polymers were, after extraction of the print molecule, successfully applied as chiral stationary phases, showing high resolution and load capacity. The development of a surface-imprinting approach for the preparation of stationary phases selective for proteins is also discussed.
用于高效液相色谱的固定相是专门为分离氨基酸、肽和蛋白质而定制的。这些固定相通过分子印迹法制备，采用了两种不同的方法。低分子量化合物通过在目标化合物（印迹分子）存在下使功能单体和交联剂共聚，在本体聚合物中印迹。在提取印迹分子后，这些聚合物成功用作手性固定相，显示出高分辨率和负载能力。还讨论了一种用于制备对蛋白质具有选择性的固定相的表面印迹方法的开发。
