Hanisch W, Meyer L V
Institut für Rechtsmedizin, München, Germany.
J Anal Toxicol. 1993 Jan-Feb;17(1):48-50. doi: 10.1093/jat/17.1.48.
A procedure has been developed for detection and quantitation of 6-monoacetylmorphine (6-MAM), the specific metabolite of heroin in human urine. 6-MAM was isolated by solid-phase extraction, and the extracts were analyzed by high-performance liquid chromatography with electrochemical detection in the oxidizing mode. Good linearity was obtained in the concentration range 10-250 ng/mL. Concentrations of 2 ng 6-MAM/mL can be detected using a 2-mL sample of urine. The method has been verified in urine samples of heroin abusers.
已开发出一种用于检测和定量人体尿液中6-单乙酰吗啡（6-MAM）的方法，6-MAM是海洛因的特定代谢物。通过固相萃取分离6-MAM，提取物采用氧化模式的电化学检测高效液相色谱法进行分析。在10-250 ng/mL的浓度范围内获得了良好的线性。使用2 mL尿液样本可检测到2 ng 6-MAM/mL的浓度。该方法已在海洛因滥用者的尿液样本中得到验证。
