Sasaki Y, Wakisaka S, Kurisu K
First Department of Oral Anatomy, Osaka University Faculty of Dentistry, Japan.
Brain Res. 1994 Nov 21;664(1-2):108-14. doi: 10.1016/0006-8993(94)91960-7.
The effects of peripheral axotomy of the inferior alveolar nerve (IAN) on the presence and distribution of neuropeptide Y (NPY)-like immunoreactivity (IR) in the trigeminal sensory nuclear complex (TSNC) and trigeminal ganglion were investigated in the rat by immunohistochemistry. In the normal trigeminal ganglion, there were no NPY-IR cells, and some perivascular nerve fibres exhibited NPY-IR. In normal TSNC, many NPY-IR axons and nerve terminals were observed in the superficial layers of the subnucleus caudalis (SpVc) and paratrigeminal nucleus (paraV), but were sparse in the other subnuclei of the TSNC. Fourteen days following peripheral axotomy of the IAN, many large- and medium-sized cells in the trigeminal ganglion displayed NPY-IR, and marked increases in the numbers and staining densities of NPY-IR were observed in deeper laminae (laminae III-V) of the dorso-medial region of the SpVc and other nuclei, in addition to the dorso-medial region of the spinal trigeminal tract. Degrees of alterations of the levels of NPY were most marked in the SpVc. The present results indicate that peripheral axotomy of the IAN evokes the appearance of NPY-IR in the trigeminal ganglion and alterations of NPY-IR in the entire IAN projection areas of the TSNC.
通过免疫组织化学方法，在大鼠中研究了下牙槽神经（IAN）外周轴突切断术对三叉神经感觉核复合体（TSNC）和三叉神经节中神经肽Y（NPY）样免疫反应性（IR）的存在和分布的影响。在正常三叉神经节中，没有NPY-IR细胞，一些血管周围神经纤维呈现NPY-IR。在正常TSNC中，在尾侧亚核（SpVc）和三叉旁核（paraV）的浅层观察到许多NPY-IR轴突和神经终末，但在TSNC的其他亚核中则很稀疏。IAN外周轴突切断术后14天，三叉神经节中的许多大中型细胞显示NPY-IR，并且除了脊髓三叉神经束的背内侧区域外，在SpVc背内侧区域的深层板层（板层III-V）和其他核中观察到NPY-IR的数量和染色密度明显增加。NPY水平的改变程度在SpVc中最为明显。目前的结果表明，IAN外周轴突切断术可引起三叉神经节中NPY-IR的出现以及TSNC整个IAN投射区域中NPY-IR的改变。
