Wakisaka S, Takikita S, Youn S H, Kurisu K
Department of Oral Anatomy and Developmental Biology, Osaka University Faculty of Dentistry, Japan.
Brain Res. 1996 Jan 29;707(2):228-34. doi: 10.1016/0006-8993(95)01262-1.
Immunohistochemistry was applied to examine the correlation between neuropeptide Y (NPY) and the two calcium binding proteins (CaBPs) parvalbumin (PV) and calbindin D28k (CB) in the trigeminal ganglion following peripheral axotomy of the inferior alveolar nerve (IAN) in the rat. Five days following transection and application of FluoroGold (FG) to the cut end of the IAN, approximately 14.8% (80/539) and 18.6% (90/483) of FG-labeled IAN neurons in the trigeminal ganglion showed PV-like immunoreactivity (-LI) and CB-LI, respectively. The mean +/- S.D. area of FG-labeled PV-like immunoreactive (-IR) cells (FG/PV-IR cells) and FG/CB-IR cells were 835.9 +/- 303.1 mu m2 and 712.7 +/- 246.0 mu m2, respectively. FG/PV-IR cells were significantly larger than FG/CB-IR cells. Fourteen days following peripheral axotomy of the IAN, NPY-LI appeared in the medium- to large-sized cells. Double immunostaining revealed that approximately 3.3% (52/1569) of NPY-IR cells in the axotomized trigeminal ganglion displayed PV-LI, while approximately 26.7% (371/1392) of NPY-IR cells displayed CB-LI. The mean +/- S.D. cross-sectional areas of PV-IR and CB-IR trigeminal ganglion cells displaying NPY-LI were 819.5 +/- 265.6 mu m2 and 766.5 +/- 279.7 mu m2, respectively. There were no significant differences in the cross-sectional areas either between NPY/PV-IR cells and NPY/CB-IR cells, or between FG/PV-IR cells and NPY/PV-IR cells, or between FG/CB-IR cells and NPY/CB-IR cells. The present results indicate that injury-evoked medium- to large-sized NPY neurons were a different population from large-sized PV neurons, and NPY was partly co-localized with CB.
应用免疫组织化学方法研究大鼠下牙槽神经（IAN）外周轴突切断后三叉神经节中神经肽Y（NPY）与两种钙结合蛋白（CaBPs）小白蛋白（PV）和钙结合蛋白D28k（CB）之间的相关性。在IAN切断并于其断端应用荧光金（FG）5天后，三叉神经节中约14.8%（80/539）和18.6%（90/483）的FG标记IAN神经元分别显示PV样免疫反应性（-LI）和CB-LI。FG标记的PV样免疫反应性（-IR）细胞（FG/PV-IR细胞）和FG/CB-IR细胞的平均±标准差面积分别为835.9±303.1μm²和712.7±246.0μm²。FG/PV-IR细胞明显大于FG/CB-IR细胞。IAN外周轴突切断14天后，NPY-LI出现在中大型细胞中。双重免疫染色显示，在切断轴突的三叉神经节中，约3.3%（52/1569）的NPY-IR细胞显示PV-LI，而约26.7%（371/1392）的NPY-IR细胞显示CB-LI。显示NPY-LI的PV-IR和CB-IR三叉神经节细胞的平均±标准差横截面积分别为819.5±265.6μm²和766.5±279.7μm²。NPY/PV-IR细胞与NPY/CB-IR细胞之间、FG/PV-IR细胞与NPY/PV-IR细胞之间以及FG/CB-IR细胞与NPY/CB-IR细胞之间的横截面积均无显著差异。目前的结果表明，损伤诱发的中大型NPY神经元与大型PV神经元是不同的群体，且NPY部分与CB共定位。
