Wakisaka S, Youn S H, Miyawaki Y, Kurisu K
Department of Oral Anatomy and Development Biology, Osaka University Faculty of Dentistry, Japan.
Brain Res. 1996 Oct 7;735(2):249-56. doi: 10.1016/0006-8993(96)00585-9.
Combined retrograde neuronal tracing with FluoroGold (FG) and a double immunofluorescence method was performed to examine the effects of peripheral nerve injury of the masseteric nerve (MassN) on the levels of two calcium binding proteins (CaBPs), parvalbumin (PV) and calbindin D28k (CB), and neuropeptide Y (NPY) in the mesencephalic trigeminal nucleus (MesV) in the rat. In the normal MesV, many medium- to large-sized unipolar PV-like immunoreactive (-IR) cells were detected through the entire rostrocaudal extent, but CB-IR cells were rarely observed. No NPY-IR cells were observed in the normal MesV. The distributions of these three neurochemical markers in the MesV contralateral to the transection of Mass were almost identical to those observed in the normal MesV. Four days following transection and application of FG to the MassN, approximately 52% (572/1104) and 38% (414/1104) of FG-labeled cells (FG cells) in the MesV displayed PV-like immunoreactivity (-LI) and NPY-LI, respectively; Approximately 24% (265/1104) of FG cells showed both PV-LI and NPY-LI. Approximately 47% (265/572) of FG cells with PV-LI showed NPY-LI or 64% (265/414) of FG cells with NPY-LI displayed PV-LI. Fourteen days following transection and application of FG, the percentage of FG cells with PV-LI significantly decreased to 36% (365/1024) compared to that observed 4 days post-injury; approximately 44% (448/1024) of FG cells displayed NPY-LI; approximately 38% (141/365) of FG cells with PV-LI showed NPY-LI and approximately 31% (141/448) of FG cells with NPY-LI displayed PV-LI. In contrast, FG cells showing CB-LI were very rare on 4 days (1%; 15/1182) or 14 days (1%; 16/1085) following MassN transection. The present results indicate that the levels of PV in the MesV decreased 14 days following the MassN injury compared to those observed 4 days post-injury and rapid induction of NPY in the injured MesV neurons, and that the correlation between CaBP and NPY in the MesV following the MassN transection is different from that observed in the trigeminal ganglion, which is equivalent to the MesV, following peripheral nerve injury of the inferior alveolar nerve.
采用逆行神经元示踪剂氟金（FG）结合双重免疫荧光法，研究大鼠咬肌神经（MassN）周围神经损伤对中脑三叉神经核（MesV）中两种钙结合蛋白（CaBPs）——小白蛋白（PV）和钙结合蛋白D28k（CB）以及神经肽Y（NPY）水平的影响。在正常MesV中，在整个前后方向上均可检测到许多中大型单极PV样免疫反应阳性（-IR）细胞，但很少观察到CB-IR细胞。在正常MesV中未观察到NPY-IR细胞。切断Mass后，MesV对侧这三种神经化学标记物的分布与正常MesV中观察到的几乎相同。切断MassN并向其施加FG后4天，MesV中约52%（572/1104）的FG标记细胞（FG细胞）显示PV样免疫反应性（-LI），约38%（414/1104）的FG细胞显示NPY-LI；约24%（265/1104）的FG细胞同时显示PV-LI和NPY-LI。约47%（265/572）具有PV-LI的FG细胞显示NPY-LI，或64%（265/414）具有NPY-LI的FG细胞显示PV-LI。切断MassN并施加FG后14天，与损伤后4天相比，具有PV-LI的FG细胞百分比显著降至36%（365/1024）；约44%（448/1024）的FG细胞显示NPY-LI；约38%（141/365）具有PV-LI的FG细胞显示NPY-LI，约31%（141/448）具有NPY-LI的FG细胞显示PV-LI。相比之下，在切断MassN后4天（1%；15/1182）或14天（1%；16/1085），显示CB-LI的FG细胞非常罕见。目前的结果表明，与损伤后4天相比，MassN损伤后14天MesV中PV水平下降，损伤的MesV神经元中NPY快速诱导，并且切断MassN后MesV中CaBP与NPY之间的相关性与下颌神经周围神经损伤后三叉神经节（相当于MesV）中观察到的不同。
