Wu W, Dai B
Hua Xi Yi Ke Da Xue Xue Bao. 1994 Sep;25(3):245-7.
Fifty-five serum samples from patients with leptospirosis treated by the method of high SiO2 concentration salt solution absorption were analysed with the variable sequences of the 16S rRNA genes of Leptospria interrogans as primers. It is shown that the 16S rRNA gene primer is useful for the clinical detection and the epidemic investigation of leptospirosis, and the detecting effect by PCR is superior to that by the blood culture and MAT (P < 0.01).
以问号钩端螺旋体16S rRNA基因的可变序列为引物，对55份采用高SiO₂浓度盐溶液吸收法治疗的钩端螺旋体病患者血清样本进行分析。结果表明，16S rRNA基因引物可用于钩端螺旋体病的临床检测和流行病学调查，且PCR检测效果优于血培养和显微镜凝集试验（P<0.01）。
