TrkH及其同源物TrkG决定了大肠杆菌Trk系统阳离子转运的特异性和动力学。
TrkH and its homolog, TrkG, determine the specificity and kinetics of cation transport by the Trk system of Escherichia coli.
作者信息
Schlösser A, Meldorf M, Stumpe S, Bakker E P, Epstein W
机构信息
Fachbereich Mikrobiologie, Universität Osnabrück, Germany.
出版信息
J Bacteriol. 1995 Apr;177(7):1908-10. doi: 10.1128/jb.177.7.1908-1910.1995.
Abstract
The corrected sequence of the trkH gene of Escherichia coli predicts that the TrkH protein is a hydrophobic membrane protein of 483 amino acid residues, of which 41% are identical to those of the homologous and functionally analogous TrkG protein. These two proteins form the transmembrane component of the Trk system for the uptake of K+. Each protein alone is sufficient for high-level Trk activity. When Trk is assembled with the TrkG protein, Rb+ and K+ are transported with a Km near or below 1 mM; however, the Vmax for Rb+ is only about 7% of that for K+. When Trk is formed with TrkH, the affinities for both for K+ and Rb+ are somewhat lower, and the Vmax for Rb+ is only 1% of that for K+ transport. The kinetics of transport in strains with wild-type alleles at trkG and at trkH suggest that both products participate in transport.
摘要
大肠杆菌trkH基因的校正序列预测，TrkH蛋白是一种由483个氨基酸残基组成的疏水性膜蛋白，其中41%与同源且功能类似的TrkG蛋白相同。这两种蛋白构成了Trk系统摄取K⁺的跨膜组分。每种蛋白单独存在时都足以实现高水平的Trk活性。当Trk与TrkG蛋白组装时，Rb⁺和K⁺的转运Km值接近或低于1 mM；然而，Rb⁺的Vmax仅约为K⁺的7%。当Trk与TrkH形成时，对K⁺和Rb⁺的亲和力均略低，且Rb⁺的Vmax仅为K⁺转运的1%。在trkG和trkH具有野生型等位基因的菌株中，转运动力学表明这两种产物均参与转运。
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本文引用的文献
1
NAD+ binding to the Escherichia coli K(+)-uptake protein TrkA and sequence similarity between TrkA and domains of a family of dehydrogenases suggest a role for NAD+ in bacterial transport.
Mol Microbiol. 1993 Aug;9(3):533-43. doi: 10.1111/j.1365-2958.1993.tb01714.x.
2
Molecular genetic analysis of a locus required for resistance to antimicrobial peptides in Salmonella typhimurium.
EMBO J. 1993 Nov;12(11):4053-62. doi: 10.1002/j.1460-2075.1993.tb06089.x.
3
A Salmonella protein that is required for resistance to antimicrobial peptides and transport of potassium.
EMBO J. 1994 Sep 1;13(17):3964-72. doi: 10.1002/j.1460-2075.1994.tb06712.x.
4
The codon preference plot: graphic analysis of protein coding sequences and prediction of gene expression.
Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):539-49. doi: 10.1093/nar/12.1part2.539.
5
Sequence of the lactose permease gene.
Nature. 1980 Feb 7;283(5747):541-5. doi: 10.1038/283541a0.
6
Replacement and amplification of bacterial genes with sequences altered in vitro.
Proc Natl Acad Sci U S A. 1983 Aug;80(16):4894-8. doi: 10.1073/pnas.80.16.4894.
7
Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes.
J Mol Biol. 1986 May 5;189(1):113-30. doi: 10.1016/0022-2836(86)90385-2.
8
A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.
Proc Natl Acad Sci U S A. 1985 Feb;82(4):1074-8. doi: 10.1073/pnas.82.4.1074.
9
Site-directed insertion and deletion mutagenesis with cloned fragments in Escherichia coli.
J Bacteriol. 1985 Mar;161(3):1219-21. doi: 10.1128/jb.161.3.1219-1221.1985.
10
K+-transport protein TrkA of Escherichia coli is a peripheral membrane protein that requires other trk gene products for attachment to the cytoplasmic membrane.
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