Fujishima H, Okamoto Y, Saito I, Tsubota K
Department of Ophthalmology, Tokyo Dental College, Chiba, Japan.
J Allergy Clin Immunol. 1995 Mar;95(3):663-7. doi: 10.1016/s0091-6749(95)70169-9.
The pathogenesis of allergic conjunctivitis is largely conjectural. We investigated the possible involvement of respiratory syncytial virus (RSV), a ubiquitous respiratory pathogen, in the development of allergic conjunctivitis through immune mechanisms. A new technique of brush cytology was used to obtain conjunctival cells from 30 patients with allergic conjunctivitis and 20 control subjects. Samples were assayed for the presence of RSV sequences with the reverse-transcription polymerase chain reaction and the nested polymerase chain reaction. Specific primers and inner primers were synthesized on the basis of RNA sequences previously identified. RSV sequences were detected in 7 of 30 (23%) patient samples and 1 of 20 (5%) control samples. Our results proved that polymerase chain reaction could detect RSV sequences in conjunctival samples. RSV may be a significant pathogenic factor in allergic conjunctivitis.
过敏性结膜炎的发病机制很大程度上是推测性的。我们通过免疫机制研究了普遍存在的呼吸道病原体呼吸道合胞病毒（RSV）在过敏性结膜炎发病过程中可能发挥的作用。采用一种新的刷片细胞学技术，从30例过敏性结膜炎患者和20例对照者中获取结膜细胞。用逆转录聚合酶链反应和巢式聚合酶链反应检测样本中RSV序列的存在情况。根据先前鉴定的RNA序列合成了特异性引物和内引物。在30例患者样本中的7例（23%）和20例对照样本中的1例（5%）检测到了RSV序列。我们的结果证明聚合酶链反应能够检测结膜样本中的RSV序列。RSV可能是过敏性结膜炎的一个重要致病因素。
