鉴定葡萄膜炎患者眼内液中的新病原体。
Identification of new pathogens in the intraocular fluid of patients with uveitis.
机构信息
Department of Virology, University Medical Center Utrecht, The Netherlands.
出版信息
Am J Ophthalmol. 2010 Nov;150(5):628-36. doi: 10.1016/j.ajo.2010.05.015. Epub 2010 Aug 5.
PURPOSE
To determine infectious causes in patients with uveitis of unknown origin by intraocular fluids analysis.
DESIGN
Case-control study.
METHODS
Ocular fluids from 139 patients suspected of infectious uveitis, but negative for herpes simplex virus, varicella-zoster virus, cytomegalovirus, and Toxoplasma gondii by polymerase chain reaction and/or antibody analysis in intraocular fluids, were assessed for the presence of 18 viruses and 3 bacteria by real-time polymerase chain reaction (PCR). The ocular fluids from 48 patients with uveitis of known etiology or with cataract were included as controls.
RESULTS
Positive PCR results were found for Epstein-Barr virus, for rubella virus, and for human herpesvirus 6 each in 1 patient and for human parechovirus in 4 patients. Of the human parechovirus-positive patients, 1 was immunocompromised and had panuveitis. The other 3 patients were immunocompetent and had anterior uveitis, all with corneal involvement.
CONCLUSIONS
Human parechovirus might be associated with infectious (kerato)uveitis.
目的
通过眼内液分析确定原因不明的葡萄膜炎患者的感染病因。
设计
病例对照研究。
方法
对聚合酶链反应（PCR）和/或眼内液抗体分析阴性的 139 例疑似感染性葡萄膜炎但单纯疱疹病毒、水痘-带状疱疹病毒、巨细胞病毒和弓形体病的患者的眼内液进行实时 PCR，以检测 18 种病毒和 3 种细菌。将 48 例已知病因或白内障的葡萄膜炎患者的眼内液作为对照。
结果
1 例患者的 EBV、风疹病毒和 HHV-6，以及 4 例患者的人副流感病毒 PCR 结果为阳性。1 例人副流感病毒阳性的患者免疫功能低下，表现为全葡萄膜炎。其他 3 例患者免疫功能正常，均为前葡萄膜炎，均伴有角膜受累。
结论
人副流感病毒可能与感染性（角）巩膜炎有关。
Zotero 插件