Kühn J E, Wendland T, Schäfer P, Möhring K, Wieland U, Elgas M, Eggers H J
Institut fuer Virologie, Universitaet zu Koeln, Germany.
J Med Virol. 1994 Dec;44(4):398-405. doi: 10.1002/jmv.1890440416.
The ratio of human cytomegalovirus (HCMV) genomes per cellular genomes in serial peripheral blood leukocyte (PBL) extracts of renal allograft recipients was quantitated by competitive nested polymerase chain reaction (PCR). Patients were also monitored for the development of acute HCMV infection by detection of HCMV pp65 antigenemia, HCMV IgM antibodies, and viruria. Compared to qualitative nested HCMV PCR, the frequency of positive PCR results in renal allograft recipients without further evidence of acute HCMV infection was significantly reduced by quantitative HCMV PCR. HCMV DNA levels > or = 1,000 copies HCMV/10(6) copies beta-globin were found to be highly indicative for the development of a clinically symptomatic HCMV infection following renal allograft transplantation. In patients treated with ganciclovir, quantitation of HCMV target sequences allowed the assessment of the efficacy of antiviral therapy.
采用竞争性巢式聚合酶链反应（PCR）对肾移植受者系列外周血白细胞（PBL）提取物中每细胞基因组的人巨细胞病毒（HCMV）基因组比率进行定量分析。通过检测HCMV pp65抗原血症、HCMV IgM抗体和病毒尿，对患者急性HCMV感染的发生情况进行监测。与定性巢式HCMV PCR相比，定量HCMV PCR显著降低了无急性HCMV感染进一步证据的肾移植受者PCR阳性结果的频率。发现HCMV DNA水平≥1000拷贝HCMV/10⁶拷贝β-珠蛋白高度提示肾移植后临床症状性HCMV感染的发生。在接受更昔洛韦治疗的患者中，HCMV靶序列的定量分析有助于评估抗病毒治疗的疗效。
