Thomson J A, Kalishman J, Hearn J P
Wisconsin Regional Primate Research Center, University of Wisconsin, Madison 53715-1299.
J Med Primatol. 1994 Aug;23(6):333-6. doi: 10.1111/j.1600-0684.1994.tb00295.x.
A nonsurgical technique for the recovery of uterine stage preimplantation embryos was developed for the common marmoset (Callithrix jacchus). In 54 flush attempts, using 19 different animals, 54 morphologically normal embryos, seven unfertilized oocytes or degenerate embryos, and five empty zonae pellucidae were recovered, giving a recovery rate of 1.0 embryo per flush or 1.2 ovulation products per flush. Because the ovarian cycles of common marmosets can be synchronized with prostaglandin PGF2 alpha, multiple marmosets can be flushed in a short period, providing age-matched embryos for controlled experiments.
已为普通狨猴(Callithrix jacchus)开发出一种用于回收子宫阶段植入前胚胎的非手术技术。在54次冲洗尝试中,使用了19只不同的动物,回收了54个形态正常的胚胎、7个未受精的卵母细胞或退化胚胎以及5个空透明带,冲洗回收率为每次冲洗1.0个胚胎或每次冲洗1.2个排卵产物。由于普通狨猴的卵巢周期可以用前列腺素PGF2α同步,因此可以在短时间内对多只狨猴进行冲洗,从而为对照实验提供年龄匹配的胚胎。