Rumio C, Morini M, Miani A, Barajon I, Castano P
University of Milan, Institute of Human Anatomy, Italy.
J Microsc. 1995 Jan;177(Pt 1):85-9. doi: 10.1111/j.1365-2818.1995.tb03536.x.
A simple device is described, which allows the range of depth of scanning to be reduced when observing thick reflecting biological samples with a confocal scanning laser microscope (CSLM). Thick histological sections of human skin and rat brain stem were mounted between two coverslips ('sandwich' style) and the optical tomography was performed from both sides by turning the 'sandwich' upside-down. The samples were impregnated using standard Golgi-Cox, 'rapid Golgi' or other silver methods. The ability to turn the 'sandwich' upside-down is particularly useful when the reflective structure inspected is deep inside the section, i.e., near the lower surface of the specimen, or when it is opaque to the laser beam or excessively reflective.
本文描述了一种简单的装置，当使用共聚焦扫描激光显微镜（CSLM）观察厚的反射性生物样本时，该装置可减少扫描深度范围。将人皮肤和大鼠脑干的厚组织切片以“三明治”形式安装在两个盖玻片之间，通过将“三明治”翻转过来从两侧进行光学断层扫描。样本采用标准的高尔基-考克斯法、“快速高尔基”法或其他银染法进行浸染。当被检查的反射结构位于切片深处，即靠近标本下表面时，或者当它对激光束不透明或反射过度时，能够将“三明治”翻转过来这一特性特别有用。
