Mügge A, Brandes R P, Böger R H, Dwenger A, Bode-Böger S, Kienke S, Frölich J C, Lichtlen P R
Division of Cardiology, Hannover Medical School, Germany.
J Cardiovasc Pharmacol. 1994 Dec;24(6):994-8. doi: 10.1097/00005344-199424060-00019.
Production/release of superoxide anions from aortic rings was measured by a modified lucigenin-enhanced chemiluminescence (CL) technique. The aortic rings were obtained from control and cholesterol-fed (1% for 12 weeks) rabbits. The CL signal was significantly increased in aortic wall of cholesterol-fed rabbits. Pretreatment with oxypurinol, an inhibitor of xanthine oxidase, had a slight but insignificant effect on the CL response produced by aortic rings from control animals but significantly reduced CL response to aortic rings from cholesterol-fed rabbits. Pretreatment with diethyldithiocarbamate (DETC), an inhibitor of intrinsic superoxide dismutase (SOD), increased the CL signal for both animal groups, but this increase was greatly aggravated in aortic rings from hypercholesterolemic rabbits. Addition of phorbol 12-myristate 13 acetate (PMA) to stimulate the respiratory burst of wall-adherent and/or resident leukocytes had only slight effect on the CL response to aortic rings from control animals but extensively stimulated photon emission of aortic rings from cholesterol-fed rabbits. These findings are in agreement with the concept that the arterial wall in hypercholesterolemia and/or atherosclerosis is under increased "oxidative stress."
采用改良的光泽精增强化学发光（CL）技术测定主动脉环中超氧阴离子的产生/释放。主动脉环取自对照兔和喂食胆固醇（1%，持续12周）的兔。喂食胆固醇的兔主动脉壁中CL信号显著增加。黄嘌呤氧化酶抑制剂别嘌呤醇预处理对对照动物主动脉环产生的CL反应有轻微但不显著的影响，但显著降低了喂食胆固醇的兔主动脉环的CL反应。内源性超氧化物歧化酶（SOD）抑制剂二乙基二硫代氨基甲酸盐（DETC）预处理使两组动物的CL信号均增加，但在高胆固醇血症兔的主动脉环中这种增加更为明显。添加佛波醇12 - 肉豆蔻酸酯13 - 乙酸酯（PMA）以刺激贴壁和/或驻留白细胞的呼吸爆发，对对照动物主动脉环的CL反应仅有轻微影响，但可广泛刺激喂食胆固醇的兔主动脉环的光子发射。这些发现与高胆固醇血症和/或动脉粥样硬化状态下动脉壁“氧化应激”增加的概念一致。
