Kerridge D, Koh T Y, Johnson A M
J Gen Microbiol. 1976 Sep;96(1):117-23. doi: 10.1099/00221287-96-1-117.
The interaction of amphotericin B methyl ester (AME) with protoplasts of Candida albicans was measured indirectly by following the incorporation of [U-14C]phenylalanine into the acid-insoluble material. The inhibitory effects of AME at the minimum inhibitory concentration were prevented by the addition of 85 mM-KCl and 45 mM-MgCl2, as shown by Liras & Lampen (1974) for Saccharomyces cerevisiae. In C. albicans, pretreatment of the yeast before anti-biotic addition was unnecessary. KCl and MgCl2 did not prevent AME from binding to the protoplast membrane. This interaction was reversed by incubating the protoplasts in the presence of the protecting salts.
两性霉素B甲酯（AME）与白色念珠菌原生质体的相互作用是通过追踪[U-¹⁴C]苯丙氨酸掺入酸不溶性物质中间接测定的。如Liras和Lampen（1974年）对酿酒酵母的研究所示，添加85 mM - KCl和45 mM - MgCl₂可防止AME在最低抑菌浓度时的抑制作用。在白色念珠菌中，在添加抗生素之前对酵母进行预处理是不必要的。KCl和MgCl₂不能阻止AME与原生质体膜结合。在保护盐存在的情况下孵育原生质体可逆转这种相互作用。
