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Action of rat liver Gal beta 1-4GlcNAc alpha(2-6)-sialyltransferase on Man beta 1-4GlcNAc beta-OMe, GalNAc beta 1-4GlcNAc beta-OMe, Glc beta 1-4GlcNAc beta-OMe and GlcNAc beta 1-4GlcNAc beta-OMe as synthetic substrates.

作者信息

Hokke C H, van der Ven J G, Kamerling J P, Vliegenthart J F

机构信息

Department of Bio-Organic Chemistry, Utrecht University, The Netherlands.

出版信息

Glycoconj J. 1993 Feb;10(1):82-90. doi: 10.1007/BF00731191.

DOI:10.1007/BF00731191
PMID:8358230
Abstract

Incubation of synthetic Man beta 1-4GlcNAc beta-OMe, GalNAc beta 1-4GlcNAc beta-OMe, Glc beta 1-4GlcNAc beta-OMe, and GlcNAc beta 1-4GlcNAc beta-OMe with CMP-Neu5Ac and rat liver Gal beta 1-4GlcNAc alpha(2-6)-sialyltransferase resulted in the formation of Neu5Ac alpha 2-6Man beta 1-4GlcNAc beta-OMe, Neu5Ac alpha 2-6GalNAc beta 1-4GlcNAc beta-OMe, Neu5Ac alpha 2-6Glc beta 1-4GlcNAc beta-OMe and Neu5Ac alpha 2-6GlcNAc beta 1-4GlcNAc beta-OMe, respectively. Under conditions which led to quantitative conversion of Gal beta 1-4GlcNAc beta-OEt into Neu5Ac alpha 2-6Gal beta 1-4GlcNAc beta-OEt, the aforementioned products were obtained in yields of 4%, 48%, 16% and 8%, respectively. HPLC on Partisil 10 SAX was used to isolate the various sialyltrisaccharides, and identification was carried out using 1- and 2-dimensional 500-MHz 1H-NMR spectroscopy.

摘要

相似文献

1
Action of rat liver Gal beta 1-4GlcNAc alpha(2-6)-sialyltransferase on Man beta 1-4GlcNAc beta-OMe, GalNAc beta 1-4GlcNAc beta-OMe, Glc beta 1-4GlcNAc beta-OMe and GlcNAc beta 1-4GlcNAc beta-OMe as synthetic substrates.
Glycoconj J. 1993 Feb;10(1):82-90. doi: 10.1007/BF00731191.
2
Purification of a Gal beta 1 to 4GlcNAc alpha 2 to 6 sialyltransferase and a Gal beta 1 to 3(4)GlcNAc alpha 2 to 3 sialyltransferase to homogeneity from rat liver.从大鼠肝脏中纯化β1,4-半乳糖基-2,6-α-唾液酸转移酶和β1,3(4)-半乳糖基-2,3-α-唾液酸转移酶至均一状态。
J Biol Chem. 1982 Nov 25;257(22):13835-44.
3
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J Biol Chem. 1987 Feb 15;262(5):2025-33.
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Determination of the specificities of rat liver Gal(beta 1-4)GlcNAc alpha 2,6-sialyltransferase and Gal(beta 1-3/4)GlcNAc alpha 2,3-sialyltransferase using synthetic modified acceptors.
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本文引用的文献

1
Synthesis of a selectively protected trisaccharide building block that is part of xylose-containing carbohydrate chains from N-glycoproteins.一种选择性保护的三糖结构单元的合成，该结构单元是来自N-糖蛋白的含木糖碳水化合物链的一部分。
Carbohydr Res. 1993 Jan 15;238:135-45. doi: 10.1016/0008-6215(93)87009-h.
2
Sialylation of glycoprotein oligosaccharides N-linked to asparagine. Enzymatic characterization of a Gal beta 1 to 3(4)GlcNAc alpha 2 to 3 sialyltransferase and a Gal beta 1 to 4GlcNAc alpha 2 to 6 sialyltransferase from rat liver.与天冬酰胺N-连接的糖蛋白寡糖的唾液酸化。来自大鼠肝脏的β-1,3(4)-半乳糖基-N-乙酰葡糖胺α-2,3-唾液酸转移酶和β-1,4-半乳糖基-N-乙酰葡糖胺α-2,6-唾液酸转移酶的酶学特性。
J Biol Chem. 1982 Nov 25;257(22):13845-53.
3
Conformation of the complex oligosaccharides of glycoproteins. A vacuum ultraviolet circular dichroism study.
J Biol Chem. 1982 Jul 25;257(14):8199-204.
4
[Synthesis of the pentasaccharide chain of the Forsman antigen].[福斯曼抗原五糖链的合成]
Carbohydr Res. 1982 Mar 1;100:143-67. doi: 10.1016/s0008-6215(00)81032-0.
5
Chemical behaviour of cytidine 5'-monophospho-N-acetyl-beta-D-neuraminic acid under neutral and alkaline conditions.
Eur J Biochem. 1984 Apr 2;140(1):203-8. doi: 10.1111/j.1432-1033.1984.tb08087.x.
6
Improved spectral resolution in cosy 1H NMR spectra of proteins via double quantum filtering.通过双量子滤波提高蛋白质的 cosy 1H NMR 谱中的光谱分辨率。
Biochem Biophys Res Commun. 1983 Dec 16;117(2):479-85. doi: 10.1016/0006-291x(83)91225-1.
7
Analysis of N-acetyl-4-O-acetylneuraminic-acid-containing N-linked carbohydrate chains released by peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase F. Application to the structure determination of the carbohydrate chains of equine fibrinogen.对肽-N4-（N-乙酰-β-葡糖胺基）天冬酰胺酶F释放的含N-乙酰-4-O-乙酰神经氨酸的N-连接碳水化合物链的分析。应用于马纤维蛋白原碳水化合物链的结构测定。
Eur J Biochem. 1989 Mar 1;180(1):101-10. doi: 10.1111/j.1432-1033.1989.tb14620.x.
8
Structures of asparagine-linked oligosaccharides of the glycoprotein fetuin having sialic acid linked to N-acetylglucosamine.胎球蛋白糖蛋白中天冬酰胺连接寡糖的结构，其中唾液酸连接到N-乙酰葡糖胺上。
Biochemistry. 1989 Jul 25;28(15):6500-12. doi: 10.1021/bi00441a051.
9
The asparagine-linked oligosaccharides on bovine fetuin. Structural analysis of N-glycanase-released oligosaccharides by 500-megahertz 1H NMR spectroscopy.牛胎球蛋白上的天冬酰胺连接寡糖。通过500兆赫1H核磁共振光谱对N-聚糖酶释放的寡糖进行结构分析。
J Biol Chem. 1988 Dec 5;263(34):18253-68.
10
Sialyl-alpha 2-6-mannosyl-beta 1-4-N-acetylglucosamine, a novel compound occurring in urine of patients with beta-mannosidosis.
J Biol Chem. 1990 Nov 15;265(32):19685-9.