Cazabonne P, Bessières M H, Pipy B, Séguéla J P
Laboratoire de Parasitologie-Mycologie, C.H.U. de Rangueil, Toulouse, France.
Microbiol Immunol. 1994;38(11):909-13. doi: 10.1111/j.1348-0421.1994.tb02146.x.
This study was carried out to evaluate the effect of excreted/secreted antigens on macrophages infected by Toxoplasma gondii. Six proteins 28, 30, 45, 58, 63 and 145 kDa were separated by different chromatographic techniques. Mouse peritoneal macrophages were treated in vitro and in vivo with these purified fractions. Penetration and proliferation assays of T. gondii in the macrophages were performed in vitro. The different antigens used did not change the rate of penetration and proliferation of the parasites. Therefore, the secreted products, which are capable of provoking an immune response, could not directly activate the macrophages. Furthermore, the secreted products were not cytotoxic and neither did they possess a visible phospholipasic activity which would have increased penetration.
本研究旨在评估排泄/分泌抗原对感染弓形虫的巨噬细胞的影响。通过不同的色谱技术分离出了六种分子量分别为28、30、45、58、63和145 kDa的蛋白质。用这些纯化组分对小鼠腹腔巨噬细胞进行了体外和体内处理。体外进行了弓形虫在巨噬细胞中的穿透和增殖试验。所使用的不同抗原并未改变寄生虫的穿透和增殖速率。因此，能够引发免疫反应的分泌产物不能直接激活巨噬细胞。此外，分泌产物没有细胞毒性，也不具有会增加穿透性的明显磷脂酶活性。
