Collery M, Delacour J, Jousselin-Hosaja M
Laboratoire de Psychophysiologie, Université Paris, France.
Neuroscience. 1994 Dec;63(3):667-77. doi: 10.1016/0306-4522(94)90513-4.
We recently found that adrenal medulla grafts implanted into the hippocampus of rats survived for several months and significantly decreased the deficits produced by hippocampal lesions in the radial maze test [Jousselin-Hossaja et al. (1994) Neuroscience 59, 275-284]. These grafts contained choline acetyltransferase immunopositive chromaffin cells and received cholinergic innervation. In the experiments reported here, adrenal medulla grafts implanted in lesioned hippocampus were treated with cholinergic differentiation factor/leukemia inhibitory factor. In the presence of this factor, the number of chromaffin cells with cholinergic phenotypes increased as well as the beneficial effects of the grafts on the performances of rats in the radial maze. These results suggest that the functional effects of adrenal medulla grafted into the hippocampus set into play cholinergic mechanisms. The cholinergic differentiation factor/leukemia inhibitory factor may also have facilitated the survival and recovery of cholinergic neurons in the host tissue. However, due to the large range of action of this cytokine and the richness of the adrenal medulla contents, non cholinergic factors are also probably involved. Our results may help to elucidate the functions of the cholinergic differentiation factor/leukemia inhibitory factor since they provide the first indication that its intracerebral injection may have behavioral effects. Moreover, our data confirm the possibility of improving the efficiency of adrenal medulla implants in the central nervous system by appropriate treatments, not only by facilitating survival but also by selectively amplifying some potential factors of the graft. This might greatly enlarge the field of this grafting technique for analysing the normal functioning of the brain and for repairing it.
我们最近发现，植入大鼠海马体的肾上腺髓质移植物存活了数月，并在放射状迷宫试验中显著减轻了海马体损伤所产生的缺陷[朱塞兰 - 霍萨亚等人（1994年），《神经科学》59卷，第275 - 284页]。这些移植物含有胆碱乙酰转移酶免疫阳性的嗜铬细胞，并接受胆碱能神经支配。在本文报道的实验中，植入受损海马体的肾上腺髓质移植物用胆碱能分化因子/白血病抑制因子进行了处理。在该因子存在的情况下，具有胆碱能表型的嗜铬细胞数量增加，并且移植物对大鼠在放射状迷宫中表现的有益作用也增强。这些结果表明，植入海马体的肾上腺髓质的功能作用启动了胆碱能机制。胆碱能分化因子/白血病抑制因子也可能促进了宿主组织中胆碱能神经元的存活和恢复。然而，由于这种细胞因子的作用范围广泛以及肾上腺髓质成分丰富，非胆碱能因子可能也参与其中。我们的结果可能有助于阐明胆碱能分化因子/白血病抑制因子的功能，因为它们首次表明其脑内注射可能具有行为学效应。此外，我们的数据证实了通过适当处理提高肾上腺髓质植入物在中枢神经系统中效率的可能性，不仅是通过促进存活，还通过选择性地放大移植物的一些潜在因子。这可能会极大地扩大这种移植技术在分析大脑正常功能和修复大脑方面的应用领域。
