Jousselin-Hosaja M, Collery M, Delacour J
Université Pierre et Marie Curie, Departement de Neurobiologie des Signaux Intercellulaires, CNRS URA 1488, Paris, France.
Neuroscience. 1994 Mar;59(2):275-84. doi: 10.1016/0306-4522(94)90595-9.
Behavioral and immunocytochemical techniques were used to study the effects of adrenal medulla grafts implanted in hippocampus--after lesion of this structure--on the memory capacities of rats. Performances of the grafted rats in the radial maze test were significantly improved and, in some aspects, fully restored. On the other hand, grafts had no significant effects on a one-trial spatial recognition test and impaired object recognition. Immunocytochemical identification showed that the grafts contained chromaffin cells with a choline acetyltransferase stainings while, in parallel, phenylethanolamine-N-methyltransferase stainings seemed to be decreased. Cholinergic innervation was established between the graft and the host hippocampus. A likely interpretation of this complex pattern of results is that the functional effects of the grafts depended on the arousal level induced by the behavioral task. At the neurobiological level, these effects probably set into play an interaction between opioid, catecholaminergic and cholinergic factors. Our results may contribute to the clarification of the problem of specificity of functional effects of intracerebral grafts as well as the problem of hippocampal role in learning and memory.
行为学和免疫细胞化学技术被用于研究在海马体损伤后将肾上腺髓质移植到该结构中对大鼠记忆能力的影响。移植大鼠在放射状迷宫试验中的表现显著改善，并且在某些方面完全恢复。另一方面，移植对单次试验空间识别试验没有显著影响，但损害了物体识别能力。免疫细胞化学鉴定表明，移植组织含有经胆碱乙酰转移酶染色的嗜铬细胞，与此同时，苯乙醇胺 -N-甲基转移酶染色似乎减少。移植组织与宿主海马体之间建立了胆碱能神经支配。对这一复杂结果模式的一种可能解释是，移植组织的功能效应取决于行为任务所诱导的唤醒水平。在神经生物学层面，这些效应可能引发了阿片类、儿茶酚胺能和胆碱能因子之间的相互作用。我们的结果可能有助于阐明脑内移植功能效应的特异性问题以及海马体在学习和记忆中的作用问题。
