Todd A J, Spike R C, Brodbelt A R, Price R F, Shehab S A
Department of Anatomy, University of Glasgow, U.K.
Neuroscience. 1994 Dec;63(3):805-16. doi: 10.1016/0306-4522(94)90525-8.
In order to determine which types of spinal neuron produce c-fos in response to noxious stimulation, we have combined pre-embedding detection of c-fos-like immunoreactivity with post-embedding immunocytochemistry using antibodies against GABA and glycine, 2 h after subcutaneous injection of formalin into a hindpaw of anaesthetized rats. Throughout the spinal cord, the majority of c-fos-immunoreactive neurons (72-81%) did not possess GABA- or glycine-like immunoreactivity, while the remaining cells contained one or both types of immunoreactivity. In the superficial dorsal horn (laminae I and II) and dorsal white matter, between 14 and 20% of c-fos-immunoreactive neurons were GABA-immunoreactive, and some of these were also glycine-immunoreactive. A single neuron in lamina I in one animal was glycine- but not GABA-immunoreactive. In the remainder of the spinal cord, between 21 and 35% of the c-fos-immunoreactive cells were GABA- or glycine-immunoreactive, and the majority of these neurons contained both types of immunoreactivity. These results suggest that some inhibitory neurons in both the superficial and deep parts of the dorsal horn are activated by noxious stimuli. It is known that some of the cells which produce c-fos in response to noxious stimulation are projection neurons, with axons ascending to the brainstem or thalamus, however, because of the large number of c-fos-immunoreactive cells in the dorsal horn, it is likely that many are interneurons, and some of these are probably excitatory cells which use glutamate as a transmitter. It therefore appears that after noxious stimulation c-fos is produced in several types of spinal neuron, including projection cells and both excitatory and inhibitory interneurons.
为了确定哪些类型的脊髓神经元在受到伤害性刺激时会产生c-fos，我们在给麻醉大鼠的后爪皮下注射福尔马林2小时后，将c-fos样免疫反应性的包埋前检测与使用抗GABA和甘氨酸抗体的包埋后免疫细胞化学相结合。在整个脊髓中，大多数c-fos免疫反应性神经元（72%-81%）不具有GABA或甘氨酸样免疫反应性，而其余细胞含有一种或两种类型的免疫反应性。在浅表背角（I层和II层）和背侧白质中，14%-20%的c-fos免疫反应性神经元是GABA免疫反应性的，其中一些也是甘氨酸免疫反应性的。在一只动物的I层中有一个神经元是甘氨酸免疫反应性的，但不是GABA免疫反应性的。在脊髓的其余部分，21%-35%的c-fos免疫反应性细胞是GABA或甘氨酸免疫反应性的，并且这些神经元中的大多数含有两种类型的免疫反应性。这些结果表明，背角浅层和深层的一些抑制性神经元会被伤害性刺激激活。已知一些在受到伤害性刺激时产生c-fos的细胞是投射神经元，其轴突向上延伸至脑干或丘脑，然而，由于背角中大量的c-fos免疫反应性细胞，很可能许多是中间神经元，其中一些可能是使用谷氨酸作为递质的兴奋性细胞。因此，在受到伤害性刺激后，似乎几种类型的脊髓神经元会产生c-fos，包括投射细胞以及兴奋性和抑制性中间神经元。
