Fleming A A, Todd A J
Department of Anatomy, University of Glasgow, UK.
Brain Res. 1994 Feb 28;638(1-2):347-51. doi: 10.1016/0006-8993(94)90670-x.
In order to determine which types of neuron in spinal dorsal horn contain the peptide TRH, pre-embedding immunocytochemistry with antiserum to TRH was combined with post-embedding detection of GABA- and glycine-like immunoreactivity. The majority (88/101) of TRH-immunoreactive neurons were also GABA-immunoreactive, but none were glycine-immunoreactive. This suggests that TRH is mainly present in inhibitory interneurons which release GABA but not glycine, and provides further evidence that there are functional differences between those GABAergic neurons in the superficial dorsal horn that contain glycine, and those that do not.
为了确定脊髓背角中哪些类型的神经元含有促甲状腺激素释放激素（TRH），将抗TRH血清的包埋前免疫细胞化学与包埋后γ-氨基丁酸（GABA）和甘氨酸样免疫反应性检测相结合。大多数（88/101）TRH免疫反应性神经元也具有GABA免疫反应性，但无一具有甘氨酸免疫反应性。这表明TRH主要存在于释放GABA而非甘氨酸的抑制性中间神经元中，并进一步证明了浅表背角中含有甘氨酸的GABA能神经元与不含有甘氨酸的GABA能神经元之间存在功能差异。
