Simmons D R, Spike R C, Todd A J
Laboratory of Human Anatomy, University of Glasgow, UK.
Neurosci Lett. 1995 Mar 3;187(2):119-22. doi: 10.1016/0304-3940(95)11358-4.
In order to determine which types of neuron in laminae I-III of the rat spinal dorsal horn contain the peptide galanin, pre-embedding immunocytochemistry with antiserum to galanin was combined with post-embedding detection of GABA- and glycine-like immunoreactivities. Sixty-eight galanin immunoreactive neurons in laminae I-III selected from four rats were examined, and in each case semi-thin sections through the cell body were tested with a monoclonal antibody to GABA and an antiserum to glycine. All of the 68 galanin-immunoreactive neurons tested were GABA-immunoreactive, while only one of them (in lamina III) was glycine-immunoreactive. This suggests that galanin is contained in inhibitory interneurons, and that (like enkephalin, neuropeptide Y and thyrotropin-releasing hormone) it is mainly restricted to GABAergic neurons which do not use glycine as a co-transmitter.
为了确定大鼠脊髓背角I-III层中哪些类型的神经元含有甘丙肽，将抗甘丙肽抗血清的包埋前免疫细胞化学与包埋后GABA和甘氨酸样免疫反应性检测相结合。对从四只大鼠中选出的I-III层中的68个甘丙肽免疫反应性神经元进行了检查，在每种情况下，用抗GABA单克隆抗体和抗甘氨酸抗血清对穿过细胞体的半薄切片进行检测。所检测的68个甘丙肽免疫反应性神经元均为GABA免疫反应性，而其中只有一个（在III层）为甘氨酸免疫反应性。这表明甘丙肽存在于抑制性中间神经元中，并且（与脑啡肽、神经肽Y和促甲状腺激素释放激素一样）它主要局限于不使用甘氨酸作为共递质的GABA能神经元。
