Blondeau K, Boze H, Jung G, Moulin G, Galzy P
Chaire de Microbiologie Industrielle et de Génétique des Micro-organismes, ENSA-INRA, Montpellier, France.
Yeast. 1994 Oct;10(10):1297-303. doi: 10.1002/yea.320101006.
Production of recombinant human serum albumin (rHSA) controlled by the constitutive promoter phosphoglycerate kinase was studied in Kluyveromyces lactis. It was governed by both cell concentration and glycolytic flow. The triggering of the fermentation metabolism by unfavourable culture conditions (pH, pO2, D) caused a decrease in the synthesis of the heterologous protein. The highest productivity (75 mg l-1 per h) and rHSA concentration (62 mg l-1) were obtained in chemostat culture with a dilution rate of 0.12 h-1 and with 38 g l-1 dry weight.
研究了在乳酸克鲁维酵母中由组成型启动子磷酸甘油酸激酶控制的重组人血清白蛋白（rHSA）的生产。它受细胞浓度和糖酵解通量的控制。不利的培养条件（pH、pO2、D）引发发酵代谢，导致异源蛋白合成减少。在稀释率为0.12 h-1且干重为38 g l-1的恒化器培养中获得了最高生产率（每小时75 mg l-1）和rHSA浓度（62 mg l-1）。
