Blondeau K, Boutur O, Boze H, Jung G, Moulin G, Galzy P
Chaire de Microbiologie Industrielle et de Génétique des Microorganismes, E.N.S.A.-I.N.R.A., Montpellier, France.
Appl Microbiol Biotechnol. 1994 May;41(3):324-9. doi: 10.1007/BF00221227.
The use of a phosphate organic form taken up by Kluyveromyces lactis removes repression of the PHO5 promoter and releases heterologous interleukin 1 beta synthesis while providing sufficient phosphate for growth. The oxidative metabolism of high-cell-density fed-batch and chemostat cultures was thus maintained under derepressed protein synthesis conditions. Interleukin 1 beta production was then growth-associated, an unusual mode of protein synthesis regulation under the control of the PHO5 promoter.
乳酸克鲁维酵母摄取的有机磷酸盐形式的使用消除了PHO5启动子的抑制作用，并释放了异源白细胞介素1β的合成，同时为生长提供了足够的磷酸盐。因此，在去抑制的蛋白质合成条件下维持了高细胞密度补料分批培养和恒化器培养的氧化代谢。白细胞介素1β的产生随后与生长相关，这是在PHO5启动子控制下一种不寻常的蛋白质合成调节模式。
