Ishikawa N, Eguchi K, Ueki Y, Nakashima M, Shimada H, Ito K, Nagataki S
Ito Hospital, Tokyo, Japan.
Clin Exp Immunol. 1993 Nov;94(2):363-70. doi: 10.1111/j.1365-2249.1993.tb03458.x.
The present study was performed to elucidate the role of adhesion molecules in the pathogenesis of Graves' disease. Peripheral blood and intrathyroidal mononuclear cells were obtained from 14 patients with Graves' disease. The expression of adhesion molecules and HLA-DR antigen on CD4+ cells and CD4+ cell subpopulations was analysed by the two- or three-colour immunofluorescence method. The expression of adhesion molecules including LFA-1 alpha, LFA-1 beta, CD2, VLA-4 alpha and VLA-5 alpha on CD4+ cells in the thyroid gland was markedly higher than that in peripheral blood. In peripheral blood CD4+ cell subsets, the CD4+ CD45RO+ cell population had an enhanced expression of the adhesion molecules compared with the CD4+ CD45RA+ cell population. However, there was no significant difference in the expression of adhesion molecules by CD4+ cell populations and subsets between Graves' disease and healthy subjects. The thyroid gland from Graves' disease contained a higher percentage of CD4+ CD45RO+ cells and a lower percentage of CD4+ CD45RA+ cells. In intrathyroidal CD4+ cell subsets, the CD4+ CD45RO+ cell population had an increased expression of LFA-1 and CD2 compared with the CD4+ CD45RA+ cell population, but there was no significant difference in VLA-4 and VLA-5 expression between the two cell subsets. Furthermore, the expression of LFA-1 and CD2 on the CD4+ CD45RO+ cell population in the thyroid was significantly higher than that in matched peripheral blood. A similar finding was also observed for the CD4+ CD45RA+ cell population. The thyroid gland had an increased percentage of CD4+ HLA-DR+ cells compared with matched or healthy peripheral blood. However, there was no significant difference in the percentage of HLA-DR+ cells in the thyroid gland between CD4+ CD45RO+ cell and CD4+ CD45RA+ cell populations. These results suggest that increased expression of adhesion molecules on CD4+ cells may be responsible for the migration of these cells into thyroid glands and cellular interactions between these cells and thyroid epithelial cells.
本研究旨在阐明黏附分子在格雷夫斯病发病机制中的作用。从14例格雷夫斯病患者获取外周血和甲状腺内单核细胞。采用双色或三色免疫荧光法分析CD4⁺细胞及CD4⁺细胞亚群上黏附分子和HLA - DR抗原的表达。甲状腺内CD4⁺细胞上包括LFA - 1α、LFA - 1β、CD2、VLA - 4α和VLA - 5α在内的黏附分子表达明显高于外周血。在外周血CD4⁺细胞亚群中，CD4⁺CD45RO⁺细胞群体与CD4⁺CD45RA⁺细胞群体相比，黏附分子表达增强。然而，格雷夫斯病患者与健康受试者的CD4⁺细胞群体及亚群的黏附分子表达无显著差异。格雷夫斯病患者的甲状腺中CD4⁺CD45RO⁺细胞百分比更高，CD4⁺CD45RA⁺细胞百分比更低。在甲状腺内CD4⁺细胞亚群中，CD4⁺CD45RO⁺细胞群体与CD4⁺CD45RA⁺细胞群体相比，LFA - 1和CD2表达增加，但两个细胞亚群的VLA - 4和VLA - 5表达无显著差异。此外，甲状腺内CD4⁺CD45RO⁺细胞群体上LFA - 1和CD2的表达明显高于配对的外周血。CD4⁺CD45RA⁺细胞群体也观察到类似结果。与配对的或健康的外周血相比，甲状腺中CD4⁺HLA - DR⁺细胞百分比增加。然而，甲状腺内CD4⁺CD45RO⁺细胞群体和CD4⁺CD45RA⁺细胞群体的HLA - DR⁺细胞百分比无显著差异。这些结果表明，CD4⁺细胞上黏附分子表达增加可能是这些细胞迁移至甲状腺以及这些细胞与甲状腺上皮细胞之间细胞相互作用的原因。
