Goto Y, Itoh M, Ohta Y, Ogawa N, Goto Y, Ohashi H
Department of Internal Medicine III, Hamamatsu University School of Medicine, Japan.
Thyroid. 1997 Aug;7(4):567-73. doi: 10.1089/thy.1997.7.567.
The possible role of B-cell growth factor (BCGF) in the abnormal activation of B cells in Graves' disease was investigated by measuring the production of BCGF by, and analyzing lymphocyte subsets of, peripheral blood cells and thyroid infiltrates from 10 patients with Graves' disease and peripheral blood cells from 9 healthy controls. Medium from peripheral blood and intrathyroidal T cells cultured in the absence or presence of phytohemagglutinin (PHA) was subjected to a bioassay with a cell line, KS-3.F10, responsive only to BCGF. The percentages of activated (DR+CD4+ and DR+CD8+) T cells in the thyroid of Graves' patients were significantly higher than those in the peripheral blood of patients or controls, whereas the percentage of naive (CD4+CD45RA+) T cells was lower. The percentage of B (CD20+) cells in peripheral blood was increased in patients compared with controls. BCGF production by T cells from the thyroid or peripheral blood of Graves' patients, in the absence or presence of PHA, was significantly greater than that by peripheral T cells from healthy controls. PHA-stimulated BCGF production by peripheral T cells of Graves' patients showed a significant negative correlation with the percentage of DR+CD8+ cells in peripheral blood, whereas spontaneous and PHA-stimulated BCGF production by intrathyroidal T cells from Graves' patients showed a positive correlation with the serum concentrations of triiodothyronine (T3) or thyroxine (T4). The different distributions of CD4+ T-cell subsets between thyroid and peripheral blood suggest an important role for intrathyroidal helper/suppressor-inducer (CD4+CD29+) cells in the increased production of BCGF and consequent exacerbation of autoimmunity in Graves' disease.
通过检测10例格雷夫斯病患者外周血细胞和甲状腺浸润细胞以及9例健康对照者外周血细胞中B细胞生长因子（BCGF）的产生情况，并分析淋巴细胞亚群，研究了BCGF在格雷夫斯病B细胞异常激活中的可能作用。将外周血和甲状腺内T细胞在有无植物血凝素（PHA）的情况下培养，然后用仅对BCGF有反应的细胞系KS-3.F10进行生物测定。格雷夫斯病患者甲状腺中活化（DR + CD4 +和DR + CD8 +）T细胞的百分比显著高于患者外周血或对照者外周血中的百分比，而幼稚（CD4 + CD45RA +）T细胞的百分比则较低。与对照相比，患者外周血中B（CD20 +）细胞的百分比增加。在有无PHA的情况下，格雷夫斯病患者甲状腺或外周血T细胞产生的BCGF明显高于健康对照者外周血T细胞产生的BCGF。格雷夫斯病患者外周血T细胞经PHA刺激产生的BCGF与外周血中DR + CD8 +细胞的百分比呈显著负相关，而格雷夫斯病患者甲状腺内T细胞自发产生和经PHA刺激产生的BCGF与血清三碘甲状腺原氨酸（T3）或甲状腺素（T4）浓度呈正相关。甲状腺和外周血中CD4 + T细胞亚群的不同分布表明，甲状腺内辅助/抑制诱导（CD4 + CD29 +）细胞在格雷夫斯病中BCGF产生增加及自身免疫加剧过程中起重要作用。
