日本人中HLA - B61的基因分型及关联分析

Genotyping and association analysis of HLA-B61 in Japanese.

作者信息

Lin L, Tokunaga K, Ogawa A, Ishikawa Y, Kashiwase K, Akaza T, Kuwata S, Tadokoro K, Juji T

机构信息

Department of Transfusion Medicine and Immunohematology Faculty of Medicine, University of Tokyo, Japan.

出版信息

Hum Immunol. 1993 Jun;37(2):95-100. doi: 10.1016/0198-8859(93)90147-s.

DOI:10.1016/0198-8859(93)90147-s

PMID:7901185

Abstract

The distribution of HLA-B61-related alleles, B4002-B4006, was examined in the Japanese population by using PCR-SSO and PCR-RFLP methods. About half of the B61-positive individuals possessed B4002 and the remaining half possessed B4006. In addition, these two major B61 alleles were separately associated with different HLA-C alleles: B4002 exhibited a strong linkage disequilibrium with Cw10, whereas B4006 was strongly associated with C blank and DR9. Amino acid residues that contribute to the serologic epitopes of the B61 group and their relationships with other HLA-B locus antigens are discussed.

摘要

采用聚合酶链反应-序列特异性寡核苷酸探针（PCR-SSO）和聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法，对日本人群中HLA-B61相关等位基因B4002 - B4006的分布情况进行了检测。约一半的B61阳性个体携带B4002，其余一半携带B4006。此外，这两个主要的B61等位基因分别与不同的HLA-C等位基因相关联：B4002与Cw10表现出强烈的连锁不平衡，而B4006与C空白型和DR9密切相关。文中讨论了构成B61组血清学表位的氨基酸残基及其与其他HLA-B位点抗原的关系。