Lin L, Tokunaga K, Nakajima F, Ishikawa Y, Kashiwase K, Tanaka H, Kuwata S, Sideltseva E W, Akaza T, Tadokoro K
Japanese Red Cross Central Blood Center, Tokyo.
Hum Immunol. 1995 May;43(1):51-6. doi: 10.1016/0198-8859(94)00120-f.
A B13 split antigen was newly identified with three alloantisera in Japanese, and two B13 split antigens were found in a Thai family. To confirm the variation of B13 and understand the correspondence between the serologic splits and the published B13 alleles, B1301 and B1302, we determined the sequences of genes coding for these B13 splits. The common Japanese B13 allele was found to be B1301, whereas another split antigen was shown to be coded by B1302. Two B13 variants identified in a Thai individual corresponded to B1301 and B1302. Moreover, 57 B13-positive samples from several ethnic groups were examined using the PCR-SSO method. Differing from previous reports, both B1301 and B1302 were found in samples from Asian populations. These two alleles were separately associated with different antigens: HLA-B1301 exhibited a strong association with A2, Cw10, DR12, and DQ7 antigens, whereas HLA-B1302 was strongly associated with A30, Cw6, DR7, and DQ2 antigens. In addition, applying the PCR-SSCP method, B1301 and B1302 could also be simply distinguished from each other.
