Griffiths A L, Middlesworth W, Goh D W, Hutson J M
Surgical Research Unit, Royal Children's Hospital, Melbourne, Australia.
J Pediatr Surg. 1993 Aug;28(8):1028-30. doi: 10.1016/0022-3468(93)90510-r.
It has been proposed that testicular descent is controlled indirectly by androgens acting on the central nervous system to mediate migration of the gubernaculum to the scrotum. Accumulating evidence suggests that the genitofemoral nerve may release a newly described neurotransmitter, calcitonin gene-related peptide (CGRP) to stimulate gubernacular motility during migration. This study aimed to determine whether exogenous CGRP could stimulate gubernacular migration in mice with complete androgen resistance (testicular feminization mouse [Tfm]). CGRP was injected into the right groin of neonatal Tfm mice at 2-day intervals until 2 weeks of age, when the length of the processus vaginalis was measured under a dissecting microscope. The processus vaginalis length in normal male littermates was 5.9 +/- 1.8 mm (mean +/- SD) while in the female it was 1.2 +/- 0.9 mm. Exogenous CGRP had no effect on either of these. In Tfm males CGRP caused a significant increase in the length of the processus vaginalis on the injected side (2.3 +/- 0.8 mm) compared with the uninjected side (1.4 +/- 1.0 mm). These results are consistent with the hypothesis that CGRP can replace, at least partially, the effect of androgens on gubernacular migration.
有人提出，睾丸下降是由雄激素作用于中枢神经系统间接控制的，以介导睾丸引带向阴囊迁移。越来越多的证据表明，生殖股神经可能释放一种新描述的神经递质，即降钙素基因相关肽（CGRP），以在迁移过程中刺激睾丸引带的运动。本研究旨在确定外源性CGRP是否能刺激完全雄激素抵抗小鼠（睾丸雌性化小鼠 [Tfm]）的睾丸引带迁移。每隔2天向新生Tfm小鼠的右腹股沟注射CGRP，直至2周龄，此时在解剖显微镜下测量鞘突的长度。正常雄性同窝小鼠的鞘突长度为5.9±1.8毫米（平均值±标准差），而雌性为1.2±0.9毫米。外源性CGRP对这两者均无影响。在Tfm雄性小鼠中，与未注射侧（1.4±1.0毫米）相比，CGRP使注射侧的鞘突长度显著增加（2.3±0.8毫米）。这些结果与CGRP至少可以部分替代雄激素对睾丸引带迁移的作用这一假设一致。
