绵羊生长激素基因的重复基因拷贝在第二个内含子中存在PvuII多态性。

The duplicated gene copy of the ovine growth hormone gene contains a PvuII polymorphism in the second intron.

作者信息

Gootwine E, Sise J A, Penty J M, Montgomery G W

机构信息

Department of Biochemistry, University of Otago, Dunedin, New Zealand.

出版信息

Anim Genet. 1993 Aug;24(4):319-21. doi: 10.1111/j.1365-2052.1993.tb00321.x.

DOI:10.1111/j.1365-2052.1993.tb00321.x

PMID:7902042

Abstract

PvuII restriction fragment length polymorphism (RFLP) was found at the growth hormone locus in sheep carrying the GH2 allele where the gene is duplicated. By restriction analysis and using the polymerase chain reaction we demonstrated that this RFLP is due to a mutation at the PvuII site located in the second intron of the 3' copy of the GH2 allele.

摘要

在携带GH2等位基因（该基因发生了重复）的绵羊的生长激素基因座处发现了PvuII限制性片段长度多态性（RFLP）。通过限制性分析并使用聚合酶链反应，我们证明这种RFLP是由于位于GH2等位基因3'拷贝第二个内含子中的PvuII位点发生了突变。